Planta Med 2003; 69(8): 733-738
DOI: 10.1055/s-2003-42776
Original Paper
Biochemistry, Physiology, in vitro-Cultures
© Georg Thieme Verlag Stuttgart · New York

A Phytochemical Study of Lignans in Whole Plants and Cell Suspension Cultures of Anthriscus sylvestris

Albert Koulman1 , Marlies E. Kubbinga1 , Sieb Batterman1 , Herman J. Woerdenbag1 , Niesko Pras1 , Jack G. Woolley2 , Wim J. Quax1
  • 1Department of Pharmaceutical Biology, GUIDE (Groningen University Institute for Drug Exploration), Groningen, The Netherlands
  • 2Natural Products Research Group, Faculty of Applied Sciences, De Montfort University, Leicester, UK
Further Information

Publication History

Received: December 20, 2002

Accepted: March 8, 2003

Publication Date:
06 October 2003 (online)


In the roots of Anthriscus sylvestris 12 different lignans were detected. Arctigenin, dimethylmatairesinol, dimethylthujaplicatin, podophyllotoxin, 7-hydroxyyatein and 7-hydroxyanhydropodorhizol have not been previously reported to be present in A. sylvestris. In the cell suspension cultures, which were initiated for this study, trace amounts of deoxypodophyllotoxin could be detected. With these cell suspension cultures we carried out feeding experiments using deoxypodophyllotoxin, yatein and, anhydropodorhizol. Yatein had a toxic effect on the cell cultures and was, like anhydropodorhizol, not converted into any detectable product. Deoxypodophyllotoxin, in contrast, was converted into podophyllotoxin, yielding significantly higher concentration than measured in whole plants.


Niesko Pras

Department of Pharmaceutical Biology

GUIDE (Groningen University Institute for Drug Exploration)

University of Groningen

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9713 AV Groningen

The Netherlands

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