Wirkung von Knochenersatzmaterialien auf die Osteoblastenproliferation

Zusammenfassung

Im Rahmen einer In-vitro-Studie an humanen Osteoblasten wurde der Einfluss verschiedener Knochenersatzmaterialien auf das Proliferationsverhalten und die Differenzierungsfähigkeit WST-2-Test und der Bestimmung der alkalischen Phosphatase untersucht. Der Vergleich der eingesetzten Materialien ergab für PepGen P-15™ die höchsten Proliferations- und Differenzierungsrate, gefolgt von Osteograf®/N, Algipore® und BioBase®. Bei BioOss® wurden die niedrigsten Werte gemessen. Mittels der vorgestellten Methodik scheint ein standardisierter Vergleich der osteokonduktiven Knochenersatzmaterialien im In-vitro-Modell möglich.

Summary

The effect of 5 different bone graft materials on the proliferation patterns of human osteoblasts were investigated in-vitro by the WST-1-Test and determination of alkaline phosphatase. The cultures were examined for cell growth behavior and morphology by phase contrast light and scanning electron microscopy. The comparison for the various materials showed the highest proliferation and differentiation rate for PepGen P-15™ followed by Osteograf®/N, Algipore® and BioBase®. BioOss® showed the lowest. The demonstrated study design seems to be beneficial for a standardized comparison of osteoconductive bone graft materials in-vitro.

Key Words

Knochenersatzmaterial - Osteoblasten - Proliferation

Key Words

Bone Graft Materials - Osteoblast - Proliferation

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