Regulation of Hepatic Glucocorticoid Receptors in Mice During Dietary Restriction

D. Dutta; R. Sharma

doi:10.1055/s-2003-41622

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2003; 35(7): 415-420
DOI: 10.1055/s-2003-41622

Original Clinical

Regulation of Hepatic Glucocorticoid Receptors in Mice During Dietary Restriction

D. Dutta¹ , R. Sharma¹

¹Department of Biochemistry, North Eastern Hill University, Shillong 793 022, India

Abstract

The specific binding of [³H] dexamethasone to its receptor, activation of the hormone-receptor complexes and DNase I digestion of nuclear bound hormone-receptor complexes were studied in the liver of mice during dietary restriction (alternate days of feeding for 3 months) compared to animals fed ad libitum. Results indicate an increase of receptor level (fmol/mg protein) in the diet-restricted (DR) animals as compared to those fed ad libitum (AL). Scatchard analyses confirm the increase in the level of receptors in DR animals, while the affinity (Kd) remained same in both groups of mice. Protein slot-blot analysis also depicts the increase of the receptor level in DR fed compared to the AL fed animals. The extent of temperature- and salt-dependent activation of receptors showed no marked difference in AL- and DR-fed mice. DNase I extraction of bound hormone-receptor complexes from nuclei revealed similar pattern of digestion in both groups of animals.

Key words

Hepatic glucocorticoid receptor - Dietary restriction - Mice

Full Text

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