Separation and Determination of Coumarins in the Root Bark of Three Citrus Plants by Micellar Electrokinetic Capillary Chromatography

Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the determination of xanthyletin and xanthoxyletin in the roots bark of three Citrus plants was developed for the first time. The electrophoresis buffer was 10 mmol/L sodium borate containing 50 mmol/L sodium dodecyl sulfate, 20 % (v/v) acetonitrile and 10 % (v/v) methanol (pH 9.8). The correlation coefficients of the calibration curves for xanthyletin and xanthoxyletin are 0.9993 and 0.9999, respectively, over the concentration ranges (16.0 - 800.0 mg/L; 8.0 - 400.0 mg/L).

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