Composition and Antimicrobial Activity of the Essential Oil of Artemisia absinthium from Croatia and France

Abstract

The essential oils obtained by steam distillation from the aerial parts of two populations of Artemisia absinthium, from France and from Croatia, were analyzed by GC and GC-MS. The oils of A. absinthium of French origin contain (Z)-epoxyocimene and chrysanthenyl acetate as major components while the oils of Croatian A. absinthium contain mainly (Z)-epoxyocimene and β-thujone. Analysis of oils before and after anthesis showed some quantitative differences. Analysis of separated leaves and flowering heads showed only few differences among these organs. As they contain no thujone, antimicrobial screening was performed on samples of French origin and showed that A. absinthium oil inhibited the growth of both tested yeasts (Candida albicans and Saccharomyces cerevisiae var. chevalieri).

References

• 1 Tan R X, Zheng W F, Tang H Q. Biologically active substances from the genus Artemisia .  Planta Med. 1998;  64 295-302
• 2 Chialva F, Doglia G, Liddle P AP. Chemotaxonomy of wormwood (Artemisia absinthum L.). 1. Composition of the essential oil of several chemotypes.  Z Lebensm-Unters Forsch. 1983;  176 363-6
• 3 Kaul V K, Nigam S S, Dhar K L. Antimicrobial activities of the essential oils of Artemisia absinthium L., Artemisia valesiaca Wall. and Artemisia vulgaris L.  Indian J Pharmacol. 1976;  38 21-2
• 4 Nin S, Arfaioli P, Bosetto M. Quantitative determination of some essential oil components of selected Artemisia absinthium plants.  J Essent Oil Res. 1995;  7 271-7
• 5 Bourrel C, Vilarem G, Michel G, Gaset A. Study of the bacteriostatic and fungistatic properties in solid media of 24 previously analyzed essential oils.  Rivista Italiana EPPOS. 1995;  6 3-12
• 6 Bourrel C, Dargent R, Vilrem G, Gaset A. Chemical analysis and fungistatic properties of some essential oils in a liquid medium. Effects on hyphal morphogenesis.  Rivista Italiana EPPOS. 1995;  6 31-42
• 7 Knobloch K, Pauli A, Iberl B, Weigand H, Weis N. Antibacterial and antifungal properties of essential oil components.  J Essent Oil Res. 1989;  1 119-28
• 8 Carta C, Moretti M DL, Peana A T. Activity of the oil of Salvia officinalis L. against Botrytis cinerea .  J Essent Oil Res. 1996;  8 399-404
• 9 Pitarokili D, Tzakou O, Kalamarakis A. Activity of the essential oil of Salvia pomifera L. ssp. calycina (Sm.) Hayek against soil borne pathogens.  J Essent Oil Res. 2002;  14 72-5
• 10 Adams R P. Identification of essential oil components by gas chromatrography/mass spectroscopy. Allured Publishing Corporation USA; 1995
• 11 Jennings W, Shibamoto T. Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. Academic Press New York; 1980
• 12 Belaiche P. Traité de Phytothérapie et d’aromathérapie, Tome I: l’aromatogramme. Maloine S.A. Editeur Paris; 1979
• 13 Masotti V, Viano J, Dherbomez M, Letourneux Y, Gaydou E M. Phytochemical and antimicrobial studies on Xylopia aethiopica .  Fitoterapia. 1998;  69 461-2