Planta Med 2002; 68(10): 912-915
DOI: 10.1055/s-2002-34933
Original Paper
Biochemistry, Physiologie, in vitro-cultures
© Georg Thieme Verlag Stuttgart · New York

In vitro Plant Regeneration from Leaf-Derived Callus of Cimicifuga racemosa

Hemant Lata1 , Ebru Bedir1 , Alana Hosick1 , Markus Ganzera1 , Ikhlas Khan1, 2 , Rita M. Moraes1
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, USA
  • 2Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS, USA
Further Information

Publication History

Received: January 25, 2002

Accepted: May 5, 2002

Publication Date:
21 October 2002 (online)


Cimicifuga racemosa (L.) Nutt., also known as Black Cohosh, is among the top 10 selling medicinal herbs in the United States. The rhizomes have been used to relieve menopausal discomfort. This plant is wild crafted and conservationists have expressed concerns with the sustainability of C. racemosa. Excised tissues from young leaves of C. racemosa were cultured on Murashige and Skoog’s medium (MS) supplemented with various concentrations of NAA and TDZ for production of callus. The optimum callus growth and maintenance was in 1.0 μM NAA plus 0.5 μM TDZ. Two-month-old calli were sub-cultured on different concentrations of cytokinins (BA, kinetin, 2ip, TDZ) or in combination with GA3 for shoot induction. The rate of shoot induction and proliferation was higher in MS media supplemented with 2.0 or 4.0 μM of TDZ. Concentrations of TDZ greater than 4.0 μM suppressed shoot growth. Adding 3.5 μM of GA3 into media containing BA increased shoot growth. The presence of GA3 with kinetin or TDZ did not affect shoot production. For rooting, shoots were transferred to MS medium with activated charcoal supplemented with various auxins (IAA, IBA and NAA), roots were noticed 20 days after transference. Activated charcoal was an essential component for vigorous rooting formation. Our results suggest that conservation of C. racemosa is possible through in vitro multiplication of leaf-derived callus.


BA:N 6-Benzyladenine

GA3:Gibberellic acid

2ip:N 6-2-Isopentenyladenine

IAA:Indole-3-acetic acid

IBA:Indole-3-butyric acid

NAA:α-Naphthaleneacetic acid


MS:Murashige and Skoog (1962)


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Rita M. Moraes

National Center for Natural Products Research

Research Institute of Pharmaceutical Sciences

School of Pharmacy

The University of Mississippi

University MS 38677


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