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DOI: 10.1055/s-2002-34932
© Georg Thieme Verlag Stuttgart · New York
Intermedeol Isolated from the Leaves of Ligularia fischeri var. spiciformis Induces the Differentiation of Human Acute Promyeocytic Leukemia HL-60 Cells
Publication History
Received: January 10, 2002
Accepted: May 5, 2002
Publication Date:
21 October 2002 (online)
Abstract
The present work was performed to investigate the effects of intermedeol on proliferation and differentiation of human leukemia-derived HL-60 cells as well as the underlying mechanisms for these effects. Intermedeol exhibited a potent antiproliferative activity against HL-60 cells. In addition, this compound was found to be a potent inducer for HL-60 cell differentiation as assessed by nitroblue tetrazolium reduction test, esterase activity assay, phagocytic activity assay, morphology change, and expression of CD14 and CD66b surface antigens. These results suggest that intermedeol induces differentiation of human leukemia cells to granulocytes and monocytes/macrophage lineage. Moreover, the expression level of c-myc was down-regulated during intermedeol-dependent HL-60 cell differentiation, whereas p21CIP1 was up-regulated. Taken together, our results suggest that intermedeol may have potential as a therapeutic agent in human leukemia.
Key words
Ligularia fischeri var. spiciformis - Compositae - intermedeol - differentiation - CD14 - CD66b - c-myc - p21CIP1 - HL-60 cells
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Dr. Kyung-Tae Lee
Department of Biochemistry, College of Pharmacy
Kyung-Hee University
Dongdaemun-Ku, Hoegi-Dong 130-701, Seoul
Korea
Email: ktlee@khu.ac.kr
Phone: +82-2-9610860
Fax: +82-2-9663885