Linear Relation between Cell Growth and Water Channels Conducted Lp in Nitellopsis

Abstract

The effect of Lp on growth of internodes of Nitellopsis obtusa (Desv. In Lois) J. Gr. was studied, with treatments of HgCl₂ and antibodies against aquaporins (anti-RD28) on the plasmalemma, using laser scanning confocal microscopy, and simultaneous monitoring of cell growth, turgor, E_m and Lp using a turgor/membrane potential probe (T-EP probe). Laser scanning confocal immunofluorescent microscopy indicated the immunoreaction of anti-RD28 on the plasma membrane. Experiments showed that, following treatment with 25 μM HgCl₂, cell growth was entirely and Lp largely inhibited, and no growth recovery was seen even when Lp was completely reversed by replacement of HgCl₂ with β-mercaptoethanol (ME). Anti-RD28 inhibited both Lp and growth of internodes of N. obtusa by about 80 %, and the possible influences on cell growth of changes in turgor, respiration and pH in cell walls, being excluded during treatment. It was confirmed experimentally that enlargement growth of internodes of N. obtusa was linearly related to cell Lp.

Abbreviations

RD28: aquaporins in plasmalemma of A. thaliana (28kD protein)

anti-RD28: antibody against fusion protein including conservative section in loop E of RD28

XET: xyloglucan endo-transglycosylase

Cell growth - Lp - Nitellopsis - T-EP probe

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Section Editor: U. Lüttge