APE/Ref-1 is Controlled by Both Redox and cAMP-Dependent Mechanisms in Rat Thyroid Cells

G. Tell; A. Pines; M. Pandolfi; A. V. D’Elia; D. Donnini; R. Lonigro; G. Manzini; D. Russo; C. Di Loreto; G. Damante

doi:10.1055/s-2002-33258

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2002; 34(6): 303-310
DOI: 10.1055/s-2002-33258

Original Basic

APE/Ref-1 is Controlled by Both Redox and cAMP-Dependent Mechanisms in Rat Thyroid Cells

G. Tell ² , A. Pines ² , M. Pandolfi ³ , A. V. D’Elia ¹ , D. Donnini ¹ , R. Lonigro ¹ , G. Manzini ² , D. Russo ⁴ , C. Di Loreto ³ , G. Damante ¹

¹Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine. M.A.T.I. Center.
²Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Università di Trieste, C.E.B.
³Dipartimento di Ricerche Mediche e Morfologiche, Università di Udine.
⁴Dipartimento di Scienze Farmacobiologiche, Università di Catanzaro

Abstract

APE/Ref-1 is a multifunctional protein possessing both redox and DNA repair functions. Through its redox activity, APE/Ref-1 controls the DNA-binding function of several transcriptional regulators (AP1, NF-κB, p53, Pax proteins). We have previously shown that APE/Ref-1 upregulates the transcriptional activity of the thyroid-specific transcription factor Pax8. In thyroid cells, APE/Ref-1 can be detected both in the nuclear and cytoplasmatic compartments. In this study regulatory mechanisms acting on APE/Ref-1 were revealed using the FRTL-5 cell line. TSH induces both cytoplasm-to-nucleus translocation and neosynthesis of APE/Ref-1 protein. Interestingly, only neosynthesis is dependent on cAMP signalling. In contrast, the cytoplasm-to-nucleus translocation is dependent on redox-mediated mechanisms. Based upon the data shown in this study and in others, a bimodal control of APE/Ref-1 by TSH can be delineated.

Key words

APE/Ref-1 - Redox Potential - Nuclear Translocation - Thyroid - cAMP

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Referenzen

References

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Prof. G. Damante

Dipartimento di Scienze e Tecnologie Biomediche, Università di Udine

Piazzale Kolbe 1 · 33100 Udine · Italy ·

Telefon: + 39 (0432) 494374.

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eMail: Gdamante@makek.dstb.uniud.it