Planta Med 2002; 68(7): 658-660
DOI: 10.1055/s-2002-32902
Letter
© Georg Thieme Verlag Stuttgart · New York

Effect of Acetazolamide on Hypericin Photocytotoxicity

Peter Solár1 , Ivan Čavarga2 , Jiřina Hofmanová3 , Mária Čekanová-Figurová1 , Pavol Miškovský4 , Peter Brezáni5 , Gabriela Hrčková6 , Alois Kozubík3 , Peter Fedoročko1
  • 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. Šafárik University, Kosˇice, Slovakia
  • 2Clinic of TB and Respiratory Diseases, Medical School Hospital of L. Pasteur, Kosˇice, Slovakia
  • 3Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
  • 4Department of Biophysics, Faculty of Sciences, P. J. Šafárik University, Kosˇice, Slovakia
  • 5Department of Medical Biology, Faculty of Medicine, P. J. Šafárik University, Kosˇice, Slovakia
  • 6Parasitological Institute of the Slovak Academy of Sciences, Kosˇice, Slovakia
Further Information

Publication History

Received: November 16, 2001

Accepted: February 9, 2002

Publication Date:
22 July 2002 (online)

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Abstract

The photodynamic action of hypericin (HYP) in vitro was evaluated using human leukemic HL-60 and lung carcinoma A549 cell lines. After illumination HYP (1 × 10-5 M) reduced the proliferation and/or survival of HL-60 and A549 cells vs. controls to almost to 0 % and 29 %, respectively. A lower concentration of HYP (1 × 10-6 M) decreased the proliferation and/or survival only in HL-60 cells. Non-cytotoxic concentrations of the carbonic anhydrase inhibitor acetazolamide (ACTZ) (1 × 10-3-1 × 10-6 M) significantly potentiated these effects of HYP (1 × 10-6M) in HL-60, but not in the A549 cell line. The highest concentration of ACTZ (1 × 10-3 M) also induced an increase of the subdiploid G0/G1 population in HYP (1 × 10-6 M) treated HL-60 cells from 14 % to 24 %. The results indicate that the photogenerated pH drop may participate in the potentiation of the photodynamic action of HYP observed in leukemia cells.

References

Dr. Peter Fedoročko

Department of Cellular and Molecular Biology

Faculty of Sciences, P.J. Šafárik University

Moyzesova 11

041 65 Košice

Slovakia

Email: fedvox@kosice.upjs.sk

Fax: +421-55-6222124