Planta Med 2002; 68(4): 311-315
DOI: 10.1055/s-2002-26749
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Anti-Inflammatory Triterpenes from Pistacia terebinthus Galls

Eva M. Giner-Larza1 , Salvador Máñez1 , Rosa M. Giner1 , M. Carmen Recio1 , José M. Prieto1 , Miguel Cerdá-Nicolás2 , José Luis Ríos1
  • 1Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Burjassot, Valencia, Spain
  • 2Departament de Patologia, Facultat de Medicina, Universitat de Valencia, Valencia, Spain
Further Information

Publication History

June 25, 2001

September 30, 2001

Publication Date:
02 May 2002 (online)

Abstract

From the galls of Pistacia terebinthus we obtained an extract that proved to be effective against chronic and acute inflammation. Now we report on the isolation and identification of three triterpenes: two tirucallane-type lanostanoids and one oleanane, which we have identified as masticadienonic acid (1), masticadienolic acid (2), and morolic acid (3), respectively. All of them showed effectiveness on the mouse ear inflammation induced by repeated applications of 12-O-tetradecanoylphorbol 13-acetate and on the phospholipase A2-induced foot paw edema. The pharmacological activity of the compounds was ratified by a histological study of the ear samples. In addition, they inhibited leukotriene B4 production in rat polymorphonuclear leukocytes stimulated with calcium ionophore A 23 187.

Abbreviations

DMSO:dimethyl sulfoxide

EPP:ethyl phenylpropiolate

HPLC-DAD:high performance liquid chromatography-diode
array detection

HTAB:hexadecyltrimethylammonium bromide

5-LOX:5-lipoxygenase

LTB4:leukotriene B4

MPLC:medium pressure liquid chromatography

MTT:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide

PGB2:prostaglandin B2

PLA2:phospholipase A2

PMNL:polymorphonuclear leukocytes

TMB:tetramethylbenzidine

TPA:12-O-tetradecanoylphorbol 13-acetate

VLC:vacuum liquid chromatography

References

Prof. Dr. J. L. Ríos

Departament de Farmacologia

Facultat de Farmàcia

Universitat de València

Avda. Vicent Andrés Estellés s/n

46100 Burjassot, Spain

Phone: /Fax: +34-963-864-973.

Email: riosjl@uv.es

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