Planta Med 2002; 68(3): 213-216
DOI: 10.1055/s-2002-23139
Original Paper
© Georg Thieme Verlag Stuttgart · New York

Muscle Relaxing Activity of Hyssopus officinalis Essential Oil on Isolated Intestinal Preparations

Mei Lu1 , Lucia Battinelli1 , Claudia Daniele1 , Cristiana Melchioni2 , Giuseppe Salvatore3 , Gabriela Mazzanti1
  • 1Department of Pharmacology of Natural Substances and General Physiology, University ”La Sapienza”, Rome, Italy
  • 2Department of Chemistry, University ”La Sapienza”, Rome, Italy
  • 3Department of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanità, Rome, Italy
Further Information

Publication History

April 3, 2001

September 22, 2001

Publication Date:
25 March 2002 (online)


The muscle relaxing activity of the essential oil of Hyssopus officinalis L. (Lamiaceae) and some of its main components (isopinocamphone, limonene and β-pinene) was studied on isolated preparations of guinea-pig and rabbit intestine. The essential oil and isopinocamphone inhibited the acetylcholine- and BaCl2-induced contractions in guinea-pig ileum in a concentration-dependent manner (IC50 42.4 μg/ml and 61.9 μg/ml to acetylcholine; 48.3 μg/ml and 70.4 μg/ml to BaCl2) whereas limonene or β-pinene left tissue contraction unchanged. In guinea-pig ileum H. officinalis essential oil also blocked the contractions induced by CaCl2. In isolated rabbit jejunum the essential oil reduced the amplitude of spontaneous movements and decreased the basal tone; neither haemoglobin, methylene blue, N ω-nitro-L-arginine methyl ester (L-NAME) or propranolol blocked the myorelaxant effect.


  • 1 Cabo J, Crespo M E, Jimenez J, Zarzuelo A. The spasmolytic activity of various aromatic plants from the province of Granada. I. The activity of the major components of their essential oils.  Plantes Médicinales et Phytothérapie. 1986;  20 213-8
  • 2 Taddei I, Giachetti D, Taddei E, Mantovani P, Bianchi E. Spasmolytic activity of peppermint, sage, and rosemary essences and their major constituents.  Fitoterapia. 1988;  59 463-8
  • 3 Lis-Balchin M, Hart S. A preliminary study of the effects of the essential oils on skeletal smooth muscle in vitro .  Journal of Ethnopharmacology. 1997;  58 183-7
  • 4 Leicester R J, Hunt R H. Peppermint oil reduces colonic spasm during endoscopy.  Lancet. 1982;  2 989
  • 5 Sparks M J, O’Sullivan P, Herrington A A, Morcos S K. Does peppermint oil relieve spasm during barium enema?.  British Journal of Radiology. 1995;  68 841-3
  • 6 Van Hellemont J. Hyssopus officinalis. In: Compendium de Phytotherapie. Association Pharmaceutique Belge Bruxelles; 1986: 207-8
  • 7 Salvatore G, D’Andrea A, Nicoletti M. A pinocamphone poor oil of Hyssopus officinalis L. var. decumbens from France (Banon).  Journal of Essential Oil Research. 1998;  10 563-7
  • 8 Mazzanti G, Battinelli L, Salvatore G. Antimicrobial properties of the linalool rich essential oil of Hyssopus officinalis L. var. decumbens (Lamiaceae).  Flavour and Fragrance Journal. 1998;  13 289-94
  • 9 Mazzanti G, Lu M, Salvatore G. Spasmolytic action of the essential oil from Hyssopus officinalis L. var. decumbens and its major components.  Phytotherapy Research. 1998;  12 S92-4
  • 10 Fieser LF, Fieser N. Reagent (A to Z).  In: Reagents for organic synthesis. Vol.1 John Wiley, Sons Inc New York; 1967: 142-3
  • 11 Tallarida R J, Murray R B. Manual of pharmacological calculation with computer programs. Springer-Verlag Berlin; 1986
  • 12 Magalhaes P JC, Criddle D N, Tavares R A, Melo E M, Mota T L, Leal-Cardoso J H. Intestinal myorelaxant and antispasmodic effects of the essential oil of Croton nepetaefolius and its constituents cineole, methyl-eugenol and terpineol.  Phytotherapy Research. 1998;  12 172-7
  • 13 Inoue R, Chen S. In: Siemen D, Hescheler J, editors Nonselective Cation Channels: Pharmacology, Physiology, and Biophysics. Basel; Birkenhäuser Verlag 1993: 261-8
  • 14 Inoue R, Isemberg G. Effect of membrane potential on acetylcholine-induced inward current in guinea-pig ileum.  Journal of Physiology. 1990;  424 57-71
  • 15 Samueli F, Bonnabello A, Grassi A. Antagonistic activity of verapamil and diltiazem against different intestinal smooth muscle stimuli. Arzneimittel-Forschung.  Drug Research. 1984;  34 181-4
  • 16 Hurwitz L, McGuffee L J, Little S A, Blumberg H. Evidence for two distinct types of potassium-activated calcium channels in intestinal smooth muscle.  Journal of Pharmacology and Experimental Therapeutics. 1980;  214 574-80
  • 17 Van Den Brouke C O, Lemli J A. Antispasmodic activity of Origanum compactum .  Planta Medica. 1980;  38 317-31
  • 18 Cox S C, Mann C M, Markham J L, Bell H C, Gustafson J E, Warmington J R, Wyllie S G. The mode of antimicrobial action of essential oil of Melaleuca alternifolia (tea tree oil).  Journal of Applied Microbiology. 2000;  88 170-5
  • 19 Ghelardini C, Galeotti N, Salvatore G, Mazzanti G. Local anaesthetic activity of the essential oil of Lavandula angustifolia .  Planta Medica. 1999;  65 700-3

Gabriela Mazzanti

Dipartimento di Farmacologia delle Sostanze Naturali e Fisiologia Generale

University ”La Sapienza„

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