New Macrocyclic Diterpenoids from Euphorbia esula

Abstract

The structures of two new macrocyclic jatrophane diterpenoid esters from the whole herb of Euphorbia esula, were established as 11,14-epoxy-3β,5α,7β,8α,9α,15β-hexaacetoxy-12-oxo-13αH-jatropha-6(17)-ene (1) and 1α,3β-diacetoxy-5α,7β-dibenzoyloxy-9,14-dioxo-11β,12α-epoxy-2α,8α,15β-trihydroxy-13βH-jatropha-6(17)-ene (2) by a combination of 1D- and 2D-NMR techniques as well as UV, IR and mass spectral data. Bioassay evaluation of all isolates against the human tumor cell lines (B16, KB, SMMC and BGC) indicated that ester 2 was cytotoxic to B16 with the IC₅₀ value being 1.81 μg/ml. In addition, the irritant activity assay indicated that both diterpenoids were inactive (ID²⁴ ₅₀ > 100 μg/ear).

Key words

Euphorbia esula L. - Euphorbiacae - jatrophane diterpenoid esters - irritation - cytotoxicity

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