Abstract
Genetic identification among cinnamon species was studied by analyzing nucleotide
sequences of chloroplast DNA from four species (Cinnamomum cassia, C. zeylanicum, C. burmannii and C. sieboldii). The two regions studied were the intergenic spacer region between the trnL 3’exon and trnF exon (trnL -trnF IGS) and the trnL intron region. We found nucleotide variation at one site in the trnL-trnF IGS, and at three sites in the trnL intron. With the sequence data from analysis of these regions, the four Cinnamomum species used in this study were correctly identified. Furthermore, single-strand
conformation polymorphism (SSCP) analysis of PCR products from the trnL-trnF IGS and the trnL intron resulted in different SSCP band patterns among C. cassia, C. zeylanicum and C. burmannii.
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Mareshige Kojoma
Izu Experimental Station for Medical Plants
National Institute of Health Sciences
Shizuoka
Japan
Fax: +81-484-62-4674
Email: kojoma@postman.riken.go.jp
