Planta Med 2001; 67(8): 768-771
DOI: 10.1055/s-2001-18350

© Georg Thieme Verlag Stuttgart · New York

In vitro Evaluation of the Antibacterial Activity of β-Triketones Admixed to Melaleuca Oils

F. Christoph1, 3 , P.-M. Kaulfers2 , E. Stahl-Biskup1,*
  • 1 Institut für Pharmazie, Pharmazeutische Biologie, Universität Hamburg, Hamburg, Germany
  • 2 Institut für Medizinische Mikrobiologie und Immunologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
  • 3 Part of the thesis
Further Information

Publication History

October 23, 2000

February 11, 2001

Publication Date:
09 November 2001 (online)


The in vitro antibacterial properties of mixtures of Australian tea tree oil and niaouli oil after adding the β-triketone complex isolated from manuka oil were tested. MIC and MBC values for four different bacteria were determined applying the broth dilution method. Both Melaleuca oil mixtures showed good antimicrobial effects against Staphylococcus aureus and Moraxella catarrhalis, exceeding the effectiveness of myrtol, which is well established in the treatment of acute and chronic bronchitis and sinusitis. The death kinetics of S. aureus were determined to draw subtle comparisons between the mixtures. The kill rate data indicated that both Melaleuca oil mixtures achieved a complete kill within 240 min.


MIC:minimal inhibitory concentration

MBC:minimal bactericidal concentration

CFU:colony forming unit

MHB:Mueller Hinton Broth

TSA:Trypticase Soy Agar

TSB:Trypticase Soy Broth


Prof. Dr. Elisabeth Stahl-Biskup

Institut für Pharmazie

Abteilung für Pharmazeutische Biologie

Universität Hamburg

Bundesstraße 45

20146 Hamburg


Fax: +49 (0)40-42838-3895

Phone: +49 (0)40-42838-3896