Planta Med 2001; 67(8): 705-708
DOI: 10.1055/s-2001-18345
Original Paper
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effect of Magnolol and Honokiol from Magnolia obovata on Human Fibrosarcoma HT-1080 Invasiveness in vitro

Hisamitsu Nagase1,*, Koji Ikeda1 , Yoshimichi Sakai2
  • 1 Department of Hygienics, Gifu Pharmaceutical University, Gifu, Japan
  • 2 Gifu Prefectural Institute of Forest Science, Mino, Gifu, Japan
Further Information

Publication History

October 31, 2000

January 28, 2001

Publication Date:
09 November 2001 (online)


We investigated the inhibitory effect of Magnolia obovata Thunb. bark ethanol extracts on human fibrosarcoma HT-1080 cells invasion in a reconstituted basement membrane [Matrigel (MG)]. We found that the effective components of the bark ethanol extracts were magnolol and honokiol, two biphenyl compounds. The extracts, magnolol and honokiol, did not affect HT-1080 cells adhesion to MG, but did inhibit HT-1080 cells migration at a high concentration (100 μM). HT-1080 cells secrete matrix metalloproteinase (MMP)-9, which degrades the extracellular matrix as a part of the invasive process. Magnolol and honokiol inhibited the activity of MMP-9, which may have been responsible, in part, for the inhibition of tumor cell invasiveness.


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Hisamitsu Nagase

Department of Hygienics

Gifu Pharmaceutical University

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Japan 502-8585


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