Abstract
”Da-Huang” (Radix et Rhizoma Rhei, medicinal rhubarb), a famous and important Traditional
Chinese Medicine, has often been confused with the adulterant species in the same
genus, Rheum. Through sequencing the trnL (UAA)/trnF (GAA) regions of chloroplast DNA of thirteen species of Rheum (three medicinal rhubarb species and ten adulterant ones), a molecular marker of
the medicinal species was found. A pair of PCR primers based on the sequences, was
thus designed, which amplified a highly specific DNA fragment in medicinal rhubarb
exclusively, and absent in the adulterants at all under an optimized PCR condition.
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Dr. Daming Zhang
Laboratory of Systematic and Evolutionary Botany
Institute of Botany
CAS
Xiangshan
Beijing 100093
People's Republic of China
Fax: +86-10-62590843
Email: zhangdm@ns.ibcas.ac.cn
