Exp Clin Endocrinol Diabetes 2001; 109(7): 374-377
DOI: 10.1055/s-2001-17410
Articles

© Johann Ambrosius Barth

Activities of 11β-hydroxysteroid dehydrogenase 2 in different regions of the intestinal tract of pigs

R. Claus 1 , S. Raab 2 , M. Lacorn 1
  • 1 FG Tierhaltung und Leistungsphysiologie, Institut für Tierhaltung und Tierzüchtung, Universität Hohenheim, Stuttgart, Germany
  • 2 F. Hoffmann-La Roche Ltd., PRBB, Basel, Switzerland
Further Information

Publication History

Publication Date:
25 September 2001 (online)

Summary:

11β-Hydroxysteroid dehydrogenase 2 (11β-HSD 2) converts active cortisol to inactive cortisone and thus modifies the availability of glucocorticoids for the target tissue. An additional function is the protection of the aldosterone receptor in mineralocorticoid-sensitive tissues such as the kidney and the gut. The occurrence of 11β-HSD 2 activity was investigated in several species. Data for the pig, however, so far are missing. The activity was determined by a radio-enzyme-assay based on the conversion of tritiated cortisol to cortisone under standardized incubation conditions in supernatants of homogenates prepared from tissues of four castrates. Tissues comprised several locations along the intestinal tract and in addition kidney, lung, muscle, heart, spleen and pancreas. Highest values of the enzyme activity were found in kidney and very low activities in lung tissue but no activity in muscle, spleen, heart and pancreas. In the gut, there was a continuous increase in enzyme activity from the duodenum (0.60 pmol × min- 1 × mg protein- 1) towards the colon with maximum values in the colon transversum (23.32 pmol × min- 1 × mg protein- 1). In the colon the activity was 10-fold higher than in jejunum and 3-fold higher compared to ileum. The activities did not differ significantly between the colon transversum and colon descendens.

References

Prof. Dr. Rolf Claus

FG Tierhaltung und Leistungsphysiologie

Institut für Tierhaltung und Tierzüchtung (470)

Universität Hohenheim

Garbenstr. 17

D-70599 Stuttgart

Germany

Phone: +49-711-459-2455

Fax: +49-711-459-2498

Email: thsekret@uni-hohenheim.de

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