Exp Clin Endocrinol Diabetes 2001; 109(6): 320-325
DOI: 10.1055/s-2001-17298
Articles

© Johann Ambrosius Barth

Gly15Gly polymorphism within the human adipocyte-specific apM-1gene but not Tyr111His polymorphism is associated with higher levels of cholesterol and LDL-cholesterol in caucasian patients with type 2 diabetes

B. Zietz 1 , N. Barth 1 , J. Schölmerich 1 , G. Schmitz 2 , A. Schäffler 1
  • 1 Department of Internal Medicine I, University of Regensburg, Germany
  • 2 Institute of Clinical Chemistry, University of Regensburg, Germany
Further Information

Publication History

Publication Date:
19 September 2001 (online)

Summary:

The recently described mutations within the human adipocyte-specific apM-1 gene might play a role in the pathogenesis of obesity, type 2 diabetes and related metabolic disorders. Design: Frequency of apM-1 gene polymorphisms and their association with metabolic parameters was evaluated in a population-based sample of 556 type 2 (316 males / 240 females) diabetic patients. PCR-based RFLP analysis was performed in blood samples. The T → G transition at nucleotide +45 within exon-2 [Gly15Gly] was detected with an allelic frequency of 0.91 for the wildtype allele and 0.09 for the mutated allele. The missense point mutation (TAC → CAC) at nucleotide +331 within exon 3 [Tyr111His] was detected with an allelic frequency of 0.97 and 0.03, respectively. These frequencies did not differ from a non-diabetic cohort examined earlier. Concerning the Gly15Gly polymorphism, the TT-genotype was found in 457 (82.2%) and the TG-genotype in 99 (17.8%), concerning the Tyr111His polymorphism, TT-genotype was found in 525 (94.4) and TC-genotype in 31 (5.6%) of type 2 diabetic patients. In TG-genotype as compared to TT-genotype significantly more patients had LDL-serum levels in high LDL-classes (<150 mg/dl: 24.4% (TG) vs. 41.4% (TT), 150mg/dl to 190mg/dl: 40.0% (TG) vs. 33.9% (TT), >190 mg/l: 35.6% (TG) vs. 25.0% (TT); p = 0.010). No differences in serum levels of lipids were found in genotype-subgroups of the Tyr111His polymorphism. Thus, Gly15Gly polymorphism of apM-1 gene might play a role in dyslipidaemia in type 2 diabetic patients.

References

Bettina ZietzM.D. 

Klinik und Poliklinik für Innere Medizin I

D-93042 Regensburg

Phone: 0049-941-944-7009

Fax: 0049-941-944-7019

Email: Bettina.Zietz@ klinik.uni-regensburg.de

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