Planta Med 2001; 67(6): 550-552
DOI: 10.1055/s-2001-16482
Letter

© Georg Thieme Verlag Stuttgart · New York

A Curcuminoid and Sesquiterpenes as Inhibitors of Macrophage TNF-α Release from Curcuma zedoaria

Mi Kyung Jang1 , Dong Hwan Sohn2 , Jae-Ha Ryu1,*
  • 1 College of Pharmacy, Sookmyung Women's University, Seoul, Korea
  • 2 College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Iksan-City, Geonbuk, Korea
Further Information

Publication History

October 18, 2000

November 19, 2000

Publication Date:
17 August 2001 (online)

Abstract

Tumor necrosis factor-α (TNF-α) is one of the major mediators produced in activated macrophages which contribute to the circulatory failure associated with septic shock. In the course of screening marketed oriental anti-inflammatory herbal drugs for TNF-α antagonistic activity, a crude methanolic extract of the rhizomes of Curcuma zedoaria exhibited significant activity. The activity-guided fractionation and repetitive chromatographic procedures with the EtOAc-soluble fraction resulted in the isolation of three active compounds. They were identified as 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (1), procurcumenol (2) and epiprocurcumenol (3) by spectral data analysis. They inhibited the production of TNF-α by lipopolysaccharide (LPS)-activated macrophages from the results of bioassay (IC50 values of 1 and 2 are 12.3 and 310.5 μM, respectively) and Western blot assay. These results imply that the traditional use of C. zedoaria rhizome as anti-inflammatory drug may be explained at least in part, by the inhibition of TNF-α production.

References

Prof. Jae-Ha Ryu

College of Pharmacy

Sookmyung Women's University

Chungpa-Dong 2-Ka

Yongsan-Ku

Seoul 140-742

Korea

Email: ryuha@sdic.sookmyung.ac.kr

Fax: +82-2-714-0745

Phone: Tel: +82-2-710-9568

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