Exp Clin Endocrinol Diabetes 2001; 109(5): 261-266
DOI: 10.1055/s-2001-16345
Articles

© Johann Ambrosius Barth

Expression of arginine vasopressin and vasopressin V1a receptor mRNA in diabetic (db/db) mice

M. Morita, Y. Kita, N. Morikawa, M. Iwami , Y. Notsu
  • Advanced Technological Platform Research Laboratory, Fujisawa Pharmaceutical Co., Ibaraki, Japan
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Publikationsverlauf

Publikationsdatum:
31. Dezember 2001 (online)

Summary:

To assess the involvement of arginine vasopressin (AVP) in genetical diabetic (db/db) mice, we examined the mRNA expression levels of AVP and vasopressin V1a receptors (V1aR) in brain and liver of db/db mice. In 10 week-old db/db mice, a significant elevation in blood sugar levels and plasma osmolality were observed, showing obvious diabetic symptoms. There was a significant increase in brain AVP mRNA levels in db/db mice. The expression level of liver V1aR mRNA in db/db mice was significantly down-regulated, presumably as a consequence of ligand-receptor interaction. This is in contrast to results that show no significant reduction in brain V1aR mRNA levels when comparing db/db and control mice. Thus, it is possible that in the progress of genetic diabetes mellitus, AVP acts in liver than in brain through V1aR.

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Dr. Yasuhiro Kita

Molecular Biological Research Laboratory

Fujisawa Pharmaceutical Co., Ltd.

5-2-3 Tokodai, Tsukuba

Ibaraki 300-2698

Japan

Telefon: +81-298-47-8611

Fax: +81-298-47-1535

eMail: Yasahirokia@po.fujisawa.co.jp

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