Planta Med 2001; 67(4): 301-305
DOI: 10.1055/s-2001-14330
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

In Vitro Anti-HIV Activity of Sulfated Cell-Wall Polysaccharides from Gametic, Carposporic and Tetrasporic Stages of the Mediterranean Red Alga Asparagopsis armata

C. Haslin1 - 3 , M. Lahaye2 , M. Pellegrini1 , J.-C. Chermann3,*
  • 1 Laboratoire de Biologie Marine Fondamentale et Appliquée, Faculté des Sciences de Luminy, Marseille, France
  • 2 Institut National de la Recherche Agronomique, Unité de Recherche sur les Polysaccharides, leurs Organisations et Interactions, Nantes, France
  • 3 INSERM U322, Unité des Retrovirus et Maladies Associées, Marseille, France
Further Information

Publication History

May 19, 2000

October 23, 2000

Publication Date:
31 December 2001 (online)

Abstract

The gametic, carposporic and tetrasporic reproductive stages from the Mediterranean red alga Asparagopsis armata contain peculiar sulfated galactans with galactose:3,6-anhydrogalactose:sulfates molar ratio of 1 : 0.01 : 1.23, 1 : 0.04 : 0.47 and 1 : 0.01 : 1.13, respectively. These water-soluble polysaccharides were studied for their in vitro activity against the human immunodeficiency virus (HIV-1). Gametic and tetrasporic galactans inhibit HIV replication at 10 and 8 μg/ml, respectively, as measured by HIV-induced syncitium formation as well as reverse transcriptase activity in cell-free culture supernatant. The carposporic polysaccharide is ineffective, even at 100 μg/ml. The maximal antiviral effect involves the presence of the polysaccharides after or during infection but not before infection. This time of action suggests an inhibition of an early step of HIV infection.

References

Dr. J. C. Chermann

INSERM U322

Unité des Retrovirus et Maladies Associées

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13273 Marseille Cedex 09

France

Email: u322@inserm-u322.univ-mrs.fr

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