Planta Med 2001; 67(4): 331-334
DOI: 10.1055/s-2001-14328
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Effects of Baicalein Isolated from Scutellaria baicalensis Radix on Adhesion Molecule Expression Induced by Thrombin and Thrombin Receptor Agonist Peptide in Cultured Human Umbilical Vein Endothelial Cells

Yoshiyuki Kimura1,*, Nobutoshi Matsushita2 , Kumi Yokoi-Hayashi2 , Hiromichi Okuda1
  • 1 2nd Department of Medical Biochemistry, School of Medicine, Ehime University, Ehime, Japan
  • 2 Pharmacology Laboratory, New Drug Research Department, High Quality-Life Research Laboratories, Bio-Medical Division, Sumitomo Metal Industries, Kyoto, Japan
Further Information

Publication History

May 5, 2000

October 10, 2000

Publication Date:
31 December 2001 (online)

Abstract

We examined the effects of various flavonoids isolated from the roots of Scutellaria baicalensis Georgi on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide (SFLLRNPNDKYEPF, TRAP) in cultured human umbilical vein endothelial cells. Thrombin and thrombin receptor agonist peptide induced endothelial leukocyte adhesion molecule-1 (ELAM-1) expression. Intercellular adhesion molecule-1 (ICAM-1) expression was also induced by thrombin, but not by TRAP. Baicalein isolated from Scutellariae Radix inhibited ELAM-1 expression induced by thrombin and thrombin receptor agonist peptide dose-dependently, with 50 % inhibitory concentrations (IC50) of 5.53 ± 1.68 μM and 2.44 ± 1.08 μM, respectively. Furthermore, baicalein inhibited thrombin-induced ICAM-1 expression with an IC50 of 9.67 ± 1.28 μM. In addition, baicalein inhibited the expressions of ELAM-1 and ICAM-1 stimulated by protein kinase C (PKC) activator phorbol myristate acetate (PMA).

References

Dr. Yoshiyuki Kimura

Second Department of Meical Biochemistry

School of Medicine

Ehime University

Shigenobu-cho

Onsen-gun

Ehime 791-0295

Japan

Email: yokim@m.ehime-u.ac.jp

Fax: +81-89-960-5256

Phone: Tel.: +81-89-960-5254