Planta Med 2001; 67(4): 385-387
DOI: 10.1055/s-2001-14321
Letter

© Georg Thieme Verlag Stuttgart · New York

Authentication of Chinese Crude Drug, Gecko, by Allele-Specific Diagnostic PCR

Zhongquan Liu1 , Yiquan Wang1,*, Kaiya Zhou1 , Demin Han1 , Xuegan Yang1 , Xunhong Liu2
  • 1 Institute of Genetic Resources, Nanjing Normal University, Nanjing, China
  • 2 Department of Chinese Medicinal Materials, Nanjing University of Traditional Chinese Medicine, Nanjing, China
Further Information

Publication History

June 15, 2000

September 10, 2000

Publication Date:
31 December 2001 (online)

Abstract

Based on the sequences of the mitochondrial 12S rRNA gene fragment of 17 samples from Gekkonidae, Salamandridae, Agamidae and Hynobiidae, respectively, a pair of allele-specific primers was designed for differentiating the Chinese medicinal material Gecko from its adulterants by PCR. The results of amplification with the primers indicate that amplicons from the templates of Gekko gecko were clearly revealed by agarose gel electrophoresis, whereas no evident amplicons were found from other species. The primers were employed to identify crude drug samples from different sources. Among a total of 9 samples, 3 were diagnosed as genuine Gecko. This result is consistent with morphological identification and DNA sequence analyses.

References

Prof. Yi-Quan Wang

Institute of Genetic Resources

College of Life Science

Nanjing Normal University

122# Ninghai Road

Nanjing, 210097

China

Email: wangyqnj@jlonline.com

Fax: 86-25-3738174