Planta Med 2001; 67(4): 384-385
DOI: 10.1055/s-2001-14320

© Georg Thieme Verlag Stuttgart · New York

Molecular Markers as a Tool to Verify Sexual and Apomictic Off-Spring of Intraspecific Crosses in Hypericum perforatum

Nicole Steck1 , Monika Messmer2 , Willi Schaffner1 , Karin Berger Bueter1,*
  • 1 Department of Pharmacy, Institute for Pharmaceutical Biology, University of Basel, Witterswil, Switzerland
  • 2 VitaPlant AG, Witterswil, Switzerland
Further Information

Publication History

June 4, 2000

August 26, 2000

Publication Date:
31 December 2001 (online)


The aim of this project was to establish RAPD markers to determine the percentage of sexual off-spring of the facultative apomictic plant species H. perforatum. We did reciprocal crosses between four different accessions (A × B, B × A, C × D, D × C) by mechanical emasculation and hand pollination. Genomic DNA of the parents and the off-spring was isolated and PCR conditions were optimized in order to obtain reproducible bands with RAPD markers. Of the 260 screened RAPD primers 127 revealed polymorphism between the parental lines of A and B, whereas 53 revealed no amplification products. Each progeny was tested for the presence of paternal bands with three primers. We found no sexual off-spring among the 22 progenies of A × B, the nine progenies of B × A and the ten progenies of D × C. However, we detected six sexual off-spring among the 45 progenies of C × D. We have proved that RAPD markers can be used to distinguish between sexual and apomictic off-spring in H. perforatum and that sexual off-spring can be obtained from intraspecific crosses. The percentage of sexual progeny might depend on the genotype of the parental lines.


Dr. K. Berger Büter

Institute of Pharmaceutical Biology

University of Basel

Benkentrasse 254

4108 Witterswil



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