Download PDF
Planta Med 2001; 67(4): 362-364
DOI: 10.1055/s-2001-14317
Letter

© Georg Thieme Verlag Stuttgart · New York

Inhibition of Prostaglandin E2 Production by Platycodin D Isolated from the Root of Platycodon grandiflorum

Yong Pil Kim1 , Eun Bang Lee2 , Seung Yeun Kim2 , Dawei Li2 , Hyun Seung Ban1 , Soon Sung Lim2 , Kuk Hyun Shin2 , Kazuo Ohuchi1,*
  • 1 Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan
  • 2 Natural Products Research Institute, Seoul National University, Seoul, Korea
Further Information

Publication History

June 2, 2000

August 19, 2000

Publication Date:
31 December 2001 (online)

    Permissions and Reprints

Abstract

Platycodin D, isolated from the root of Platycodon grandiflorum A. DC. (Campanulaceae) suppressed prostaglandin E2 production at 10 and 30 μM in rat peritoneal macrophages stimulated by the protein kinase C activator 12-O-tetradecanoylphorbol 13-acetate (TPA). Platycodin D3 and oleanolic acid showed no effect at these concentrations. Western blot analysis revealed that the induction of COX-2 protein by TPA was inhibited by platycodin D in parallel with the inhibition of prostaglandin E2 production. Platycodin D showed no direct effect on COX-1 and COX-2 activities. TPA-induced release of [3 H]arachidonic acid from pre-labeled macrophages was also not inhibited by platycodin D.

References

  • 1 Takagi K, Lee E B. Acute toxicity and central depressant activity of crude platycodin.  Nippon Yakugaku Zasshi. 1972;  92 951-60
    PubMedGoogle Scholar
  • 2 Tada A, Kaneiwa Y, Shoji J, Shibata S. Isolation and the structure of platycodin D.  Chem. Pharm. Bull.. 1975;  23 2965-72
    PubMedGoogle Scholar
  • 3 Yamada M, Niki H, Yamashita M, Mue S, Ohuchi K. Prostaglandin E2 production dependent upon cyclooxygenase-1 and cyclooxygenase-2 and its contradictory modulation by auranofin in rat peritoneal macrophages.  J. Pharmacol. Exp. Ther.. 1997;  281 1005-12
    PubMedGoogle Scholar
  • 4 Ishii H, Tori K, Tozyo T, Yoshimura Y. Structures of polygalacin D and D2, platycodin D and D2 and their monoacetates, saponins isolated from Platycodon grandiflorum A. D.C., determined by 13C nuclear magnetic resonance spectroscopy.  Chem. Pharm. Bull.. 1978;  26 674-7
    PubMedGoogle Scholar
  • 5 Ohuchi K, Watanabe M, Yoshizawa K, Tsurufuji S, Fujiki H, Suganuma M, Sugimura T, Levine L. Stimulation of prostaglandin E2 production by 12-O-tetradecanoylphorbol 13-acetate (TPA)-type and non-TPA-type tumor promoters in macrophages and its inhibition by cycloheximide.  Biochim. Biophys. Acta. 1985;  834 42-7
    PubMedGoogle Scholar
  • 6 Nishizuka Y. Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C.  Science. 1992;  258 607-14
    CrossrefPubMedGoogle Scholar
  • 7 Kim Y P, Yamada M, Lim S S, Lee S H, Ryu N, Shin K H, Ohuchi K. Inhibition by tectorigenin and tectoridin of prostaglandin E2 production and cyclooxygenase-2 induction in rat peritoneal macrophages.  Biochim. Biophys. Acta. 1999;  1438 399-407
    PubMedGoogle Scholar

Professor Kazuo Ohuchi, Ph.D.

Laboratory of Pathophysiological Biochemistry

Graduate School of Pharmaceutical Sciences

Tohoku University

Aoba Aramaki, Aoba-ku

Sendai, Miyagi 980-8578

Japan

Email: ohuchi-k@mail.pharm.tohoku.ac.jp

Fax: +81-22-217-6859

      >