Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs
in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine
into smaller molecules that were effectively absorbed in mice. The homogenate from
the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed
on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded
fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation.
Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose
and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate
on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and
12 × hexose on TOF-MS.
Aloemannan -
Aloe vera
- Asphodelaceae - fluoresceinyl isothiocyanate aloemannan - metabolism - degraded
fluoresceinyl thiocyanate aloemannan - fast atom bombartment mass spectrometry - matrix-assisted
laser desorption/ionization time-of-flight mass spectrometry