Planta Med 2001; 67(4): 297-300
DOI: 10.1055/s-2001-14314
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Biodisposition of FITC-Labeled Aloemannan in Mice

Akira Yagi1,*, Shinya Hamano1 , Tetsuro Tanaka1 , Yoshiharu Kaneo1 , Toshihiro Fujioka2 , Kunihide Mihashi1
  • 1 Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Japan
  • 2 Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan
Further Information

Publication History

May 5, 2000

August 9, 2000

Publication Date:
31 December 2001 (online)

Abstract

Biodisposition of FITC-labeled aloemannan (F-AM) with the homogenate from some organs in mice was demonstrated. F-AM was metabolized only by the mucosa from the large intestine into smaller molecules that were effectively absorbed in mice. The homogenate from the other tissues did not affect the metabolism of F-AM. The degraded product (1) of F-AM after incubation with 10 % feces homogenate for 24 h was chromatographed on a highly porous polymer and a Sephadex LH-20 column to provide an FITC-degraded fraction (2), which was shown to have a molecular weight of 800 D on Sephadex G-25 gel permeation. Metabolite 2 was examined by physicochemical methods and shown to be a mixture of FITC-hexose and -2 hexose on FAB-MS. An FITC-degraded fraction (3) with a molecular weight of 3 KD was obtained by 6-h incubation with 10 % feces homogenate on Sephadex G-25 column chromatography and was shown to be a mixture of FITC-9 and 12 × hexose on TOF-MS.

References

Prof. Akira Yagi

Faculty of Pharmacy and Pharmaceutical Sciences

Fukuyama University

Fukuyama

Hiroshima 729-0292

Japan

Email: yagi@fupharm.fukuyama-u.ac.jp

Fax: +81-849/36-2024