Planta Med 2001; 67(4): 379-381
DOI: 10.1055/s-2001-14310
Letter

© Georg Thieme Verlag Stuttgart · New York

Differentiation of Lycium barbarum from its Related Lycium Species using Random Amplified Polymorphic DNA

Kalin Y. B. Zhang1 , H. W. Leung1 , H. W. Yeung1 , Ricky N. S. Wong1, 2,*
  • 1 Institute for the Advancement of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
  • 2 Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
Further Information

Publication History

June 8, 2000

July 29, 2000

Publication Date:
31 December 2001 (online)

Abstract

The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.

References

Dr. Ricky N. S. Wong

Department of Biology

Hong Kong Baptist University

Kowloon Tong

Hong Kong

China

Email: rnswong@hkbu.edu.hk

Fax: 852-2339-5995