Planta Med 2001; 67(4): 376-378
DOI: 10.1055/s-2001-14308
Letter

© Georg Thieme Verlag Stuttgart · New York

Rapid Micro-Assay of Camptothecin in Camptotheca acuminata

Beth A. Nolte, R. Daniel Lineberger, David W. Reed, Mary E. Rumpho*
  • Department of Horticultural Sciences, Texas A&M University, College Station, Texas, USA
Further Information

Publication History

June 6, 2000

July 20, 2000

Publication Date:
31 December 2001 (online)

Abstract

A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.

Abbreviations

CPT:camptothecin

DCM:dichloromethane

HPLC:high performance liquid chromatography

TLC:thin-layer chromatography

TOPI:topoisomerase I

References

Mary E. Rumpho, , PhD 

Department of Biochemistry, Microbiology and Molecular Biology

5735 Hitchner Hall, Rm. 183

University of Maine

Orono

ME 04469-5735

U.S.A.

Email: mrumpho@umit.maine.edu

Fax: +1-207-581-2801