Planta Med 2001; 67(4): 374-376
DOI: 10.1055/s-2001-14307

© Georg Thieme Verlag Stuttgart · New York

Evidence for Paclitaxel from Three New Endophytic Fungi of Himalayan Yew of Nepal

Kanti Shrestha1,*, Gary A. Strobel2 , Satya Prakash Shrivastava3 , Mohan B. Gewali1
  • 1 Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • 2 Department of Plant Sciences, Montana State University, Bozeman, Montana, U.S.A.
  • 3 Analytical Chemistry Section, Industrial Toxicology Research Center, Lucknow, India
Further Information

Publication History

May 5, 2000

July 17, 2000

Publication Date:
31 December 2001 (online)


Taxus wallichiana, collected from the Himalayan region of Nepal, yielded, among others, three different endophytic fungi including Sporormia minima, Trichothecium sp., and an unidentified dimorphic fungus. Each was shown to produce paclitaxel in a culture medium. Each of these fungi represents a new report as a paclitaxel producer. The production of paclitaxel was confirmed by an immunoassay that utilized monoclonal antibodies, TLC chromatography as well as high performance liquid chromatography combined with on-line mass spectrometry.


Kanti Shrestha 


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