Planta Med 2000; 66(4): 314-317
DOI: 10.1055/s-2000-8537
Original Paper
Georg Thieme Verlag Stuttgart · New York

Suppression of Rat Neutrophil Reactive Oxygen Species Production and Adhesion by the Diterpenoid Lactone Andrographolide

Yuh-Chiang Shen1 , Chieh-Fu Chen1, 2 , Wen-Fei Chiou1,*
  • 1 National Research Institute of Chinese Medicine, Taipei, Taiwan
  • 2 Institutes of Pharmacology, School of Life Science, National Yang-Ming University, Taipei, Taiwan
Further Information

Publication History

Publication Date:
31 December 2000 (online)

Abstract

The present study was to examine whether andrographolide, a diterpenoid lactone isolated from the anti-inflammatory herbal medicine Andrographis paniculata (Burm. f.) Nees. (Acanthaceae), has the ability to prevent phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production, as well as N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced adhesion by rat neutrophils. Results demonstrated that PMA (100 ng/ml) induced rapid accumulation of H2O2 and O2 · in neutrophils within 30 minutes. Andrographolide (0.1 to 10 μM) pretreatment (10 min, 37 °C) significantly attenuated the accumulation of these two oxygen radical metabolites. Administration of andrographolide also significantly prevented fMLP-induced neutrophil adhesion. These data suggest that preventing ROS production and neutrophils adhesion may confer andrographolide the ability to be an anti-inflammatory drug.

Abbreviations

PMA:phorbol-12-myristate-13-acetatefMLP:N-formyl-methionyl-leucyl-phenylalanineROS:reactive oxygen species

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PhD Dr. Wen-Fei Chiou

National Research Institute of Chinese medicine

No. 155-1 Section 2, Li-Nung Street

Shih-Pai

Taipei

Taiwan 112, R.O.C.

Email: wfchiou@cma23.nricm.edu.tw

Phone: 886-2-28264266

Fax: 886-2-28201999 (4481)

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