Planta Med 2000; 66(1): 44-47
DOI: 10.1055/s-2000-11117
Original Paper
Georg Thieme Verlag Stuttgart · New York

Antiproliferative Effect on Human Prostate Cancer Cells by a Stinging Nettle Root (Urtica dioica) Extract

Lutz Konrad1,*, Hans-Helge Müller2 , Corinna Lenz1 , Helge Laubinger1 , Gerhard Aumüller1, and, Johannes Josef Lichius3
  • 1 Department of Anatomy and Cell Biology, Philipps-Universität Marburg, Marburg, Germany
  • 2 Institute for Medical Biometry and Epidemiology, Philipps-Universität Marburg, Marburg, Germany
  • 3 Institute of Pharmaceutical Biology, Philipps-Universität Marburg, Marburg, Germany
Further Information

Publication History

Publication Date:
31 December 2000 (online)

Abstract

In the present study the activity of a 20 % methanolic extract of stinging nettle roots (Urtica dioica L., Urticaceae) on the proliferative activity of human prostatic epithelial (LNCaP) and stromal (hPCPs) cells was evaluated using a colorimetric assay. A concentration-dependent and significant (p < 0.05) antiproliferative effect of the extract was observed only on LNCaP cells during 7 days, whereas stromal cell growth remained unaltered. The inhibition was time-dependent with the maximum of growth reduction (30 %) at a concentration of 1.0E-6 mg/ml on day 5 compared to the untreated control. On day 4 and 6, the reduction in proliferation of LNCaP cells showed the minimal effective dose at 1.0E-9 mg/ml. No cytotoxic effect of ME-20 on cell proliferation was observed. The antiproliferative effect of ME-20 of stinging nettle roots observed both in an in vivo model and in an in vitro system clearly indicates a biologically relevant effect of compounds present in the extract.

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Dr. Lutz Konrad

Department of Anatomy and Cell Biology

Philipps-University

Robert-Koch-Str. 6

D-35033 Marburg

Germany

Email: [email protected]

Phone: +49-(6421)-28-65783

Fax: +49-(6421)-28-64004