Planta Med 2000; 66(1): 7-10
DOI: 10.1055/s-2000-11112
Original Paper
Georg Thieme Verlag Stuttgart · New York

Evidence for Specific Interactions between Kavain and Human Cortical Neurons Monitored by Fluorescence Correlation Spectroscopy

Georg Boonen1, 2 , Aladdin Pramanik1 , Rudolf Rigler1, and, Hanns Häberlein1, 3,*
  • 1 Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
  • 2 Present address: Max Zeller Söhne Ltd., Romanshorn, Switzerland
  • 3 Present address: Department of Pharmaceutical Biology, Philipps-University, Marburg, Germany
Further Information

Publication History

Publication Date:
31 December 2000 (online)

Abstract

Specific interactions between a tetramethylrhodamine-labeled kavain derivative and human cortical neurons were found for the first time using fluorescence correlation spectroscopy (FCS). Human cortical neurons, cultivated in Nunc® chambers, were incubated with 1 nM of the dye-labeled kavain derivative. A total binding of 0.55 nM was found after an incubation period of 60 min. 50 % of the total binding was specifically displaced in the presence of 1 μM non-labeled (+)-kavain. Evidence for these specific interactions was verified by a saturation experiment. Both the non-linear Scatchard plot and the n value of 1.58 ± 0.07 in the sigmoid Hill plot indicate binding sites with different binding affinities.

References

Dr. Hanns Häberlein

Institut für Pharmazeutische Biologie

Philipps-Universität Marburg

Deutschhausstraße 17A

D-35032 Marburg

Germany

Email: haeberlh@mailer.uni-marburg.de

Phone: +49-6421-2825369