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Planta Med 1999; 65(5): 404-407
DOI: 10.1055/s-1999-14084
Original Paper

Georg Thieme Verlag Stuttgart · New York

Flavonoids from Cleome droserifolia Suppress NO Production in Activated Macrophages in Vitro

Shinji Fushiya1 , Yoji Kishi1 , Koichi Hattori1 , Javzan Batkhuu1 , Fumihide Takano1 , A. N.B. Singab2 , Toru Okuyama3
  • 1Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-Ku, Sendai, Japan
  • 2Department of Pharmacognosy, Faculty of Pharmacy, AL-Azhar University, Nasr City, Cairo, Egypt
  • 3Meiji Pharmaceutical University, Nosio, Kiyose, Tokyo, Japan
Further Information

Publication History

October 30,1998

January 31,1999

Publication Date:
31 December 1999 (online)

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Abstract:

The effect of an Egyptian medicinal plant, Cleome droserifolia (Forssk.) Del. on nitric oxide (NO) produrtion in bacillus Calmette-Cuerin-induced moiise peritoneal macrophages arti-vated by lipopolysaccharide was investigated in vitro. The methanoi extract of C. droserifolia reduced the NO produrtion, and two flavonoids were isolated as the active components. The new one was determined to be 5,4′-dihydroxy-6,7,8,3 ′,5′-pentamethoxyflavone ( 1) and the other was identified as 5,4′-dihydroxy-6,7,8,3 ′-tetramethoxyflavone (8-methoxycirsilineol; 2). Compound 1 concentration-dependently suppressed the NO production and was effective at a non-toxic concentration (12.5 μ/ml). The suppressive activity of 2 was weaker than that of 1.

Key words:

Cleome droserifolia - Capparidaceae - flavonoid - nitric oxide - inflammation - macrophage