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Planta Med 1999; 65(2): 157-160
DOI: 10.1055/s-1999-14058
Original Paper

Georg Thieme Verlag Stuttgart · New York

Random Primed Polymerase Chain Reaction Differentiates Codonopsis pilosula from Different Localities

Yan-Bo Zhang1,2 , Fai-Ngor Ngan2 , Zheng-Tao Wang1 , Tzi-Bun Ng2,3 , Paul Pui-Hay  But2,4 , Pang-Chui Shaw2,3 , Jung Wang2,3
  • 1Department of Pharmacognosy, School of Chinese Materia Medica, China Pharmaceutical University, Nanjing, P. R. China
  • 2Chinese Medicinal Material Research Center, The Chinese University of Hong Kong, Shatin, Hong Kong, P. R. China
  • 3Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, P. R. China
  • 4Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, P. R. China
Further Information

Publication History

June 16, 1998

September 19, 1998

Publication Date:
31 December 1999 (online)

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Abstract:

DNA fingerprints distinctive among the samples from different localities in China were successfully reproduced for the Chinese herb Dangshen, the roots of Codonopsis pilosula, (Campanulaceae). Similarity index (S.I.) analysis revealed that C. pilosula samples from the same province generated similar DNA fingerprints, while samples of different provinces displayed different DNA fingerprints. This method may be a general and valuable tool for locality authentication of other Chinese herbal medicinal materials.

Key words:

Authentication - Codonopsis pilosula - Campanulaceae - polymerase chain reaction - AP-PCR - RAPD - locality

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