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Planta Med 1999; 65(5): 400-403
DOI: 10.1055/s-1999-14014
Original Paper

Georg Thieme Verlag Stuttgart · New York

Scopoletin: An Inducible Nitric Oxide Synthesis Inhibitory Active Constituent from Artemisia feddei

Tai-Hyun Kang1 , Hyun-Ock Pae2 , Sei-Joon Jeong2 , Ji-Chang Yoo2 , Byung-Min Choi2 , Chang-Duk Jun2 , Hun-Taeg Chung2 , T. Miyamoto3 , R. Higuchi3 , Youn-Chul Kim1
  • 1College of Pharmacy, Wonkwang University, Iksan, Korea
  • 2Wonkwang University School of Medicine and Medicinal Resources Research Center, Iksan, Korea
  • 3Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
Further Information

Publication History

September 22,1998

December 26,1998

Publication Date:
31 December 1999 (online)

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Abstract:

Bioassay-guided fractionation of an H2O extract of Artemisia feddei has furnished an inducible nitric oxide synthase (iNOS) inhibitory coumarin, scopoletin (1) and one of the inactive sesquiterpenes, achillin (2). Compound 1 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-γ (IFN-γ) plus lipopolysaccharide (LPS). The Inhibition of NO synthesis of 1 was due to suppression of iNOS mRNA and iNOS protein, as determined by Northern and Western blotting, respectively.

Key words:

Artemisia feddei - Compositae - scopoletin - nitric oxide - inducible nitric oxide synthesis