Vasodilatory Effect in Rat Aorta of Eriodictyol Obtained from Satureja obovata

Abstract:

The vasodilator effect of eriodictyol (5,7,3^′,4^′ -tetrahydroxyflavanone), isolated previously from the medicinal plant Satureja obovata Lag., was studied in rat thoracic aorta rings. Eriodictyol relaxed in a concentration-dependent manner the noradrenaline (10^-6 M) and KCl (80 mM) induced contractions. The relaxant effect was more potent in noradrenaline precontracted preparations (IC₅₀ = 6.11 ± 0.2 × 10^-5 M) than in those precontracted with KCl (IC₅₀ = 2.96 ± 0.1 × 10^-4 M). Eriodictyol produced weakly concentration-dependent inhibition of the phasic component induced by KCl and noradrenaline while the inhibition of the tonic phase of these contractions was more pronounced. These effects were endothelium independent. In addition, eriodictyol (10 ^-5 and 5 × 10^-5 M) inhibited CaCl ₂ cumulative concentration response curves. Eriodictyol weakly inhibited the release of calcium from the sarcoplasmic reticulum and its contribution to the relaxant effect seems to be slight. We have also observed the relaxant effect of eriodictyol on phorbol-12-myristate-13-acetate (PMA) (10^-7 M) induced contractions both in normal calcium (IC₅₀ = 4.69 ± 0.3 × 10^-5 M) and calcium-free medium (IC₅₀ = 3.74 ± 0.4 × 10^-5 M). Finally we studied the effects on protein kinase C (PKC) activity. This flavonoid did not show any activity. These results suggest that the vasodilator effect of eriodictyol in rat thoracic aorta could be partially related to the inhibition of calcium influx or other enzymatic protein subsequent to activation of PKC related to the activation of contractile proteins like myosin light chain kinase (MLCK).

Key words:

Eriodictyol - flavonoid - Satureja obovata - Lamiaceae - rat aorta - vascular smooth muscle relaxant - PKC activity