Planta Med 1999; 65(2): 120-125
DOI: 10.1055/s-1999-13971
Original Paper

Georg Thieme Verlag Stuttgart · New York

Antiviral Activities of Biflavonoids

Yuh-Meei Lin1 , Michael T. Flavin1 , Ralph Schure1 , Fa-Ching Chen2 , Robert Sidwell3 , Dale I. Barnard3 , John H. Huffmann3 , Earl R. Kern4
  • 1MediChem Research, Lemont, Illinois, U.S.A.
  • 2Department of Chemistry, National Taiwan University, Taipei, Taiwan
  • 3Institute of Antiviral Research, Utah State University, Logan, Utah, U.S.A.
  • 4Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, U.S.A.
Further Information

Publication History

April 30, 1998

September 9, 1998

Publication Date:
31 December 1999 (online)


Biflavonoids such as amentoflavone (1), agathisflavone (2), robustaflavone (3), hinokiflavone (4), volkensiflavone (5), rhusflavanone (7), succedaneaflavanone (9), all isolated from Rhus succedanea and Garcinia multiflora, as well as their methyl ethers and acetates, volkensiflavone hexamethyl ether (6), rhusflavanone hexaacetate (8), and succedaneaflavanone hexaacetate ( 10) were evaluated for their antiviral activities. The inhibitory activities against a number of viruses including respiratory viruses (influenza A, influenza B, respiratory syncytial, parainfluenza type 3, adenovirus type 5, and measles) and herpes viruses (HSV-1, HSV-2, HCMV, and VZV) were investigated. The results indicated that robustaflavone exhibited strong inhibitory effects against influenza A and influenza B viruses with EC50 values of 2.0 μg/ml and 0.2 μg/ml, respectively, and selectivity index values (SI) of 16 and 454, respectively. Amentoflavone and agathisflavone also demonstrated significant activity against influenza A and B viruses. Amentoflavone and robustaflavone exhibited moderate anti-HSV-1 and anti-HSV-2 activities with EC50 values of 17.9 μg/ml (HSV-1) and 48.0 μg/ml (HSV-2) and SI values of > 5.6 (HSV-1) and > 2.1 (HSV-2) for amentoflavone; EC50 values of 8.6 μg/ml (HSV-1) and 8.5 μg/ml (HSV-2), and SI values of > 11.6 (HSV-1) and > 11.8 (HSV-2) for robustaflavone. Rhusflavanone demonstrated inhibitory activities against influenza B, measles, and HSV-2 viruses with SI values of 9.3, 8 and > 6.4, respectively. Succedaneaflavanone exhibited inhibitory activities against influenza B virus and VZV with SI values of 15 and <3.0, respectively.