Abstract
Objectives
The purpose of this study was to analyze the application of chitosan (CH)-Aloe vera (AV) gel on blood vessels, vascular endothelial growth factor (VEGF), and fibroblast
growth factor (FGF) expression in traumatic ulcer healing.
Materials and Methods
Chitosan powder with deacetylation degree of 93% dissolved in 2% acetic acid, neutralized
with NaOH solution, and combined with AV gel. Traumatic ulcers were made with a 4-mm
diameter along the lower labial mucosa of Wistar rats using a heated burnisher. Negative
control group (KA) was treated with placebo, positive control group (KB) using 0.2%
hyaluronic acid, treatment group using chitosan (CH) gel (PA) and AV gel (PB), and
group using combination CH-AV gel (PC). The blood vessel numbers were analyzed with
hematoxylin eosin staining and the expression of VEGF and FGF were analyzed using
immunohistochemistry on days 3 and 7.
Statistical Analysis
One-way analysis of variance was conducted to analyze the difference between groups
and the Tukey honestly significant difference multiple comparisons test was used to
analyze different pairs of groups (p < 0.05).
Results
Blood vessels, VEGF, and FGF expression were higher in the treatment group using combination
CH-AV gel on days 3 and 7 compared with the control group and treatment group using
CH or AV gel (p < 0.05).
Conclusion
The application of CH-AV gel increased the number of blood vessels, VEGF, and FGF
expression in traumatic ulcer healing of Wistar rats.
Keywords
chitosan -
Aloe vera
- traumatic ulcer healing - blood vessel - VEGF - FGF
