Subscribe to RSS

DOI: 10.1055/s-0045-1810015
Apical Positioned Flap with a Combination of Mucograft and Fibro-Gide for Augmenting Keratinized Gingiva with Thin Gingival Phenotype: A Case Report
Funding This work was supported by a general program grant from the Beijing Haidian Hospital (No. KYM2025003) and was partially supported by a key program grant from the Beijing Haidian Hospital (No. KYZ2025002).

Abstract
The width and thickness of keratinized gingiva play a crucial role in the periodontal health and long-term stability of natural teeth. Noncrosslinked xenogeneic collagen matrix (Mucograft) has demonstrated favorable outcomes in augmenting keratinized gingival width (KGW), though its effect on increasing gingival thickness remains suboptimal. In contrast, crosslinked xenogeneic collagen matrix (Fibro-Gide) effectively enhances keratinized gingival thickness (KGT) but is unable to increase KGW. The aim of this study is to investigate the combined transplantation of Mucograft and Fibro-Gide in patients with thin gingival biotypes and insufficient KGW, evaluating whether this approach can simultaneously achieve both widening and thickening of the keratinized gingiva. This study selected a patient with a thin gingival biotype and insufficient KGW. A horizontal incision was made 0.5 mm above the mucogingival junction, and a partial-thickness flap was elevated and repositioned apically. Fibro-Gide was placed at the apical aspect of the surgical site, enveloped by the partial-thickness flap, while its coronal aspect was positioned in close contact with Mucograft, which was exposed in the surgical area. Both graft materials were securely sutured to the periosteum, ensuring partial contact with the keratinized gingiva. At the 12-month follow-up, the KGW increased from 1.5 to 3.5 mm and the KGT increased from 0.8 to 2.1 mm. For patients with a thin gingival biotype, the combined transplantation of Mucograft and Fibro-Gide can simultaneously augment the width of keratinized gingiva and increase gingival thickness.
Key Points
This technique has the following advantages:
-
Reduces the need of harvesting autologous soft tissue grafts.
-
Minimal postoperative discomfort for the patient.
-
Widens and thickens keratinized gingiva at the same time, and enhances the effect of XCM in patients with thin gingival phenotype.
Keywords
Mucograft - Fibro-Gide - keratinized gingiva - thin gingival phenotype - apical positioned flapEthical Approval and Consent to Participate
This study was conducted in accordance with the Declaration of Helsinki and approved by the Clinical Research Ethics Committee of the Beijing Haidian Hospital (IRB approval number: BHHMEC-XM-2022–21).
Consent for Publication
Written informed consent for publication was obtained from the patient to publish all clinical data and accompanying images.
Authors' Contributions
Y.L. conceived the experiments, and X.R.Z. has performed the surgery and collected the data.
Publication History
Article published online:
18 July 2025
© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India
-
References
- 1 Gharpure AS, Latimer JM, Aljofi FE, Kahng JH, Daubert DM. Role of thin gingival phenotype and inadequate keratinized mucosa width (<2 mm) as risk indicators for peri-implantitis and peri-implant mucositis. J Periodontol 2021; 92 (12) 1687-1696
- 2 Kan JY, Morimoto T, Rungcharassaeng K, Roe P, Smith DH. Gingival biotype assessment in the esthetic zone: visual versus direct measurement. Int J Periodontics Restorative Dent 2010; 30 (03) 237-243
- 3 Wessel JR, Tatakis DN. Patient outcomes following subepithelial connective tissue graft and free gingival graft procedures. J Periodontol 2008; 79 (03) 425-430
- 4 Rokn A, Zare H, Haddadi P. Use of Mucograft Collagen Matrix® versus free gingival graft to augment keratinized tissue around teeth: a randomized controlled clinical trial. Front Dent 2020; 17 (05) 1-8
- 5 Schmitt CM, Moest T, Lutz R, Wehrhan F, Neukam FW, Schlegel KA. Long-term outcomes after vestibuloplasty with a porcine collagen matrix (Mucograft® ) versus the free gingival graft: a comparative prospective clinical trial. Clin Oral Implants Res 2016; 27 (11) e125-e133
- 6 Qiu X, Li X, Li F. et al. Xenogeneic collagen matrix versus free gingival graft for augmenting keratinized mucosa around posterior mandibular implants: a randomized clinical trial. Clin Oral Investig 2023; 27 (05) 1953-1964
- 7 Clem D, McClain P, McGuire M. et al. Harvest graft substitute for soft tissue volume augmentation around existing implants: A randomized, controlled and blinded multicenter trial. J Periodontol 2024; 95 (08) 740-748
- 8 Thoma DS, Alshihri A, Fontolliet A, Hämmerle CHF, Jung RE, Benic GI. Clinical and histologic evaluation of different approaches to gain keratinized tissue prior to implant placement in fully edentulous patients. Clin Oral Investig 2018; 22 (05) 2111-2119
- 9 Ahmedbeyli C, Ipçi SD, Cakar G, Kuru BE, Yılmaz S. Clinical evaluation of coronally advanced flap with or without acellular dermal matrix graft on complete defect coverage for the treatment of multiple gingival recessions with thin tissue biotype. J Clin Periodontol 2014; 41 (03) 303-310
- 10 Ramanauskaite A, Obreja K, Müller KM. et al. Three-dimensional changes of a porcine collagen matrix and free gingival grafts for soft tissue augmentation to increase the width of keratinized tissue around dental implants: a randomized controlled clinical study. Int J Implant Dent 2023; 9 (01) 13
- 11 Solonko M, Regidor E, Ortiz-Vigón A, Montero E, Vilchez B, Sanz M. Efficacy of keratinized mucosal augmentation with a collagen matrix concomitant to the surgical treatment of peri-implantitis: a dual-center randomized clinical trial. Clin Oral Implants Res 2022; 33 (01) 105-119
- 12 Fu X, Wang Y, Chen B, Tian J, Lin Y, Zhang Y. Patient-reported outcome measures and clinical outcomes following peri-implant vestibuloplasty with a free gingival graft versus xenogeneic collagen matrix: a comparative prospective clinical study. Int J Implant Dent 2021; 7 (01) 69
- 13 Ashurko I, Tarasenko S, Esayan A. et al. Connective tissue graft versus xenogeneic collagen matrix for soft tissue augmentation at implant sites: a randomized-controlled clinical trial. Clin Oral Investig 2022; 26 (12) 7191-7208
- 14 Papace C, Büsch C, Ristow O, Keweloh M, Hoffmann J, Mertens C. The effect of different soft-tissue management techniques for alveolar ridge preservation: a randomized controlled clinical trial. Int J Implant Dent 2021; 7 (01) 113
- 15 Cosyn J, Eeckhout C, Christiaens V. et al. A multi-centre randomized controlled trial comparing connective tissue graft with collagen matrix to increase soft tissue thickness at the buccal aspect of single implants: 3-month results. J Clin Periodontol 2021; 48 (12) 1502-1515
- 16 Wennström JL, Bengazi F, Lekholm U. The influence of the masticatory mucosa on the peri-implant soft tissue condition. Clin Oral Implants Res 1994; 5 (01) 1-8
- 17 Monje A, Blasi G. Significance of keratinized mucosa/gingiva on peri-implant and adjacent periodontal conditions in erratic maintenance compliers. J Periodontol 2019; 90 (05) 445-453
- 18 da Silva DM, Castro F, Martins B, Fraile JF, Fernandes JCH, Fernandes GVO. The influence of the gingival phenotype on implant survival rate and clinical parameters: a systematic review. Evid Based Dent 2025
- 19 Alagappan A, Muthu J, Ravindran S, Balu P, Ramkumar N, Arulanandan S. Collagen matrix versus free gingival graft for augmentation of keratinized tissue in mandibular anterior teeth: a comparative clinical study. J Indian Soc Periodontol 2024; 28 (06) 685-690
- 20 Strasding M, Sailer I, Merino-Higuera E. et al. A novel pre-customized saddle-shape soft tissue substitute for volume augmentation: an ex vivo study in pig mandibles. Materials (Basel) 2025; 18 (09) 1951
- 21 Vallecillo C, Toledano-Osorio M, Vallecillo-Rivas M, Toledano M, Osorio R. In vitro biodegradation pattern of collagen matrices for soft tissue augmentation. Polymers (Basel) 2021; 13 (16) 2633
- 22 Toledano M, Toledano-Osorio M, Carrasco-Carmona Á. et al. State of the art on biomaterials for soft tissue augmentation in the oral cavity. Part I: natural polymers-based biomaterials. Polymers (Basel) 2020; 12 (08) 1850
- 23 Fazekas R, Molnár B, Kőhidai L. et al. Blood flow kinetics of a xenogeneic collagen matrix following a vestibuloplasty procedure in the human gingiva-An explorative study. Oral Dis 2019; 25 (07) 1780-1788
- 24 Froum SJ, Khouly I, Tarnow DP. et al. The use of a xenogeneic collagen matrix at the time of implant placement to increase the volume of buccal soft tissue. Int J Periodont Restor Dent 2015; 35 (02) 179-189
- 25 Wang Q, Meng HX, Song WL. et al. Short-term clinical efficacy of a collagen matrix and free gingival graft in augmenting keratinized mucosa around dental implants. Chung Hua Kou Chiang Hsueh Tsa Chih 2019; 54 (02) 79-86
- 26 Alrayyes Y, Aloraini S, Alkhalaf A, Aljasser R. Soft-tissue healing assessment after extraction and socket preservation using platelet-rich fibrin (PRF) in smokers: a single-blinded, randomized, controlled clinical trial. Diagnostics (Basel) 2022; 12 (10) 2403
- 27 Schmitt CM, Tudor C, Kiener K. et al. Vestibuloplasty: porcine collagen matrix versus free gingival graft: a clinical and histologic study. J Periodontol 2013; 84 (07) 914-923