A aplicação tópica de nanocápsulas de estrogênio na incisão cutânea melhora a Consolidação de fraturas em ratas osteoporóticas

Resumo

Objetivo O desafio de consolidação de fraturas osteoporóticas, particularmente exacerbadas pela deficiência de estrogênio pós-menopausa, ressalta a necessidade urgente de intervenções eficazes. Este estudo tem como objetivo avaliar o impacto do estrogênio administrado localmente via nanocápsulas na consolidação de fraturas osteoporóticas em ratas ovariectomizadas e analisar os efeitos sistêmicos deste hormônio usando o útero como órgão sentinela.

Métodos Quarenta e cinco animais foram submetidos a fraturas femorais padronizadas e divididos em três grupos: G1 (controle), G2 (estrogênio convencional) e G3 (nanocápsulas de estrogênio). O estrogênio foi aplicado topicamente na região da incisão da pele (área tricotomizada). A cicatrização da fratura foi avaliada 15 e 30 dias após a fratura por meio de análises radiográficas e histológicas. A histologia uterina analisou os efeitos sistêmicos.

Resultados Na análise radiográfica dos calos ósseos, G3 (8,75 ± 0,77 mm) exibiu formação de calo significativamente maior do que o grupo controle (7,18 ± 0,4 mm) no dia 15 e a análise histológica revelou aumento da formação de calo em G3 no dia 30, indicando um processo de cicatrização acelerado. Além disso, a análise histológica uterina no dia 30 mostrou uma redução na espessura endometrial em G3 (510.073 ± 54.705,11 μm) em comparação a G2 (623.729 ± 101.592 μm).

Conclusão Estes achados sugerem que nanocápsulas tópicas de estrogênio podem aumentar a formação de calos no tratamento de fraturas femorais osteoporóticas em ratas, potencialmente com menos efeitos sistêmicos.

Abstract

Objective The challenge of consolidating osteoporotic fractures, particularly exacerbated by postmenopausal estrogen deficiency, underscores the urgent need for effective interventions. This study aims to evaluate the impact of locally administered estrogen via nanocapsules on the consolidation of osteoporotic fractures in ovariectomized rats, while also assessing the systemic effects of this hormone, using the uterus as a sentinel organ.

Methods Forty-five animals underwent standardized femoral fractures and were divided into three groups: G1 (control), G2 (conventional estrogen), and G3 (estrogen nanocapsules). The estrogen was applied topically to the skin incision region (trichotomized area). Fracture healing was assessed at 15- and 30-days postfracture through radiographic and histological analyses, with uterine histology conducted to evaluate systemic effects.

Results In terms of radiographic analysis of callus formation, G3 (8.75 ± 0.77 mm) exhibited significantly higher callus formation than the control group (7.18 ± 0.4 mm) at day 15, with histological analysis revealing increased callus formation in G3 at day 30, indicating an accelerated healing process. Furthermore, uterine histological analysis at day 30 showed a reduction in endometrial thickness in G3 (510,073 ± 54,705.11 μm) compared with G2 (623,729 ± 101,592 μm).

Conclusion These findings suggest that topical estrogen nanocapsules may enhance callus formation in the treatment of osteoporotic femoral fractures in rats, potentially with fewer systemic effects.

Palavras-chave

consolidação da fratura - estrogênios - nanotecnologia - osteoporose

Keywords

estrogens - fracture healing - nanotechnology - osteoporosis

Suporte Financeiro

Os autores declaram que não receberam suporte financeiro de agências dos setores público, privado ou sem fins lucrativos para a realização deste estudo.

Trabalho desenvolvido no Departamento de Medicina, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brasil.

Publikationsverlauf

Eingereicht: 09. Juli 2024

Angenommen: 02. Oktober 2024

Artikel online veröffentlicht:
14. Juni 2025

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