Phyllanthin from Phyllanthus amarus exerts neuroprotective effects against spinal cord injury in experimental rats

Abstract

Background

Spinal cord injury (SCI) results in changes in autonomic function, impacting an individual's movement, sensory perception, and overall quality of life. Phyllanthin, a lignan from Phyllanthus amarus, is known for its neuronal protective effects.

Objective

To evaluate the potential of phyllanthin identified in P. amarus methanolic extract (PAME) against SCI in experimental rats.

Methods

The lignan was identified in PAME using high-performance liquid chromatography (HPLC). Spinal cord injury was induced in Sprague-Dawley rats using the laminectomy clip compression method. Rats received either a vehicle (distilled water) or methylprednisolone (30 mg/kg) or PAME (50, 100, and 200 mg/kg) orally for 4 weeks after SCI. Behavioral, histological, and molecular parameters were assessed to evaluate the neuroprotective effects of phyllanthin.

Results

During the HPLC analysis of PAME, phyllanthin was present at a retention time of 25.30 minutes with 75.22% weight per weight (w/w). The administration of standardized PAME (100 and 200 mg/kg) effectively ameliorated the alterations induced by SCI in thermal and mechano-tactile hyperalgesia, locomotor activity, and nerve conduction velocity (p < 0.05 each). The SCI-induced elevation in spinal interleukins (ILs: IL-1β and IL-6) and tumor necrosis factor alpha (TNF-α) protein levels was also effectively (p < 0.05) reduced by PAME. The PAME treatment markedly (p < 0.05) ameliorated SCI-induced alterations in protein expressions of B-cell leukemia/lymphoma 2 (Bcl-2), Bcl2-associated X protein (Bax), and caspase-3 in the spinal cord. Aberrations, such as inflammatory infiltration, edema, congestion, and necrosis induced in the spinal cord, were also effectively reduced by the PAME treatment (p < 0.05).

Conclusion

Phyllanthin identified in P. amarus showed neuroprotective potential against SCI by moderating impairments in behavioral (allodynia, hyperalgesia, and nerve conduction velocity) parameters, elevated inflammatory mediators (IL-1β, IL-6, and TNF-α), and deactivating the apoptotic signaling (Bax/caspase-3) pathway.

Keywords

Apoptosis - Inflammation - Phyllanthus - Spinal Cord Injuries

Authors' Contributions

Conceptualization: JE, ZF; Data curation: JE; Formal analysis: YC, YY; Investigation: YC; Supervision: YY; Writing - original draft: JE, YC, YY, ZF; Writing - review & editing: JE, YC, YY,ZF.

Data Availability Statement

Available based upon reasonable request.

Editor-in-Chief: Hélio A. G. Teive 0000-0003-2305-1073.

Associate Editor: Luciene Covolan 0000-0002-9751-6438.

Publication History

Received: 21 January 2025

Accepted: 19 March 2025

Article published online:
17 June 2025

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