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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0045-1809182
Original Article

Performance Validation of Fabricated Nanomaterial-Based Biosensor for Matrix Metalloproteinase-8 Protein Detection

Autor*innen

  • Pimsunee Lowpradit

    1   Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

  • Rapiphun Janmanee

    2   Department of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, Thailand

  • Kallapat Tansriratanawong 

    1   Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

Funding This work was funded by the National Science Research and Innovation Fund (NSRF), 2568A14212049. This work was also supported in part by the Faculty of Dentistry, Mahidol University and the Department of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University.
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Abstract

Objective

Matrix metalloproteinase-8 (MMP-8) is a crucial collagenase enzyme that primarily degrades type I collagen and extracellular glycoproteins, playing a significant role in the pathological processes of periodontal disease. It can serve as a biomarker for early detection and screening of the disease through advanced biosensor technology. The aim of this study was to fabricate and validate the performance of a nanomaterial-based biosensor for detecting MMP-8 protein.

Materials and Methods

The screen-printed gold electrode was modified with a thin film of 11-mercaptoundecanoic acid using the self-assembled monolayer technique. A biosensor was then created with N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to form ester bonds, followed by the immobilization of the antibody MMP-8 and blocking of nonspecific binding. The performance characteristics of a biosensor for detecting MMP-8 concentrations, ranging from 1 to 50 ng/mL, were evaluated using electrochemical techniques with data analysis performed using the NOVA software. Enzyme-linked immunosorbent assay (ELISA) was used as a control.

Statistical Analysis

The results were expressed as mean values ± standard deviation. The coefficient of determination (R 2) was calculated based on the obtained calibration curve.

Results

Electrochemical measurements revealed that the peak current after modifying the thin film on the electrode was lower than the bare electrode. Characterization of biosensors showed an increase in response compared to the previous step. Differential pulse voltammetry measurements indicated that the peak current for MMP-8 concentrations ranging from 1 to 50 ng/mL increased proportionally with concentration. The biosensor demonstrated high sensitivity, with a correlation coefficient of R 2 = 0.953 when compared to ELISA (R 2 = 1).

Discussion and Conclusion

A biosensor utilizing nanomaterials has been successfully fabricated for the detection of the MMP-8 protein with high sensitivity. Subsequent research should prioritize the evaluation of its performance in clinical patients alongside an assessment of its specificity and stability. The objective was to advance this biosensor as a reliable diagnostic tool for the screening of periodontitis.

Keywords

biosensor - nanomaterials - matrix metalloproteinase-8 - periodontitis - point-of-care testing


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Artikel online veröffentlicht:
21. Mai 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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