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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0045-1808261
Original Article

Chemical Elements and Thickness of Candida albicans Biofilm Induced by Glucose, Lactose, Protein, and Iron

Indah Listiana Kriswandini 
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Sidarningsih Sidarningsih 
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Prawati Nuraini 
2   Departement of Pedodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nur Mega Dwi Y. 
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Mohammed Ahmed Aljunaid 
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
3   Department of Oral and Dental Medicine, Faculty of Medicine, Taiz University, Taiz, Yemen
,
Oki Fadhila 
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
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Abstract

Objective

Health is the most important aspect that needs to be considered, and the oral cavity cannot be separated from other parts. Candida albicans is a normal flora in the oral cavity that is a major cause of oral candidiasis. Research on biofilms can help prevent oral candidiasis infection in the community. Biofilms are involved in the pathogenesis and could be examined using an electron and fluorescence microscope, which can analyze the whole biofilm in actual conditions. This study aims to determine the chemical elements and thickness of Candida albicans biofilms induced by glucose, lactose, soy protein, and iron.

Materials and Methods

This analytic observational study was carried out by observing the chemical elements and thickness of the biofilm by scanning electron microscopy with energy dispersive X-ray (SEM-EDX) and confocal laser scanning microscopy (CLSM). SEM-EDX data analysis used the EDAX APEX software and CLSM used the Olympus FluoView ver 4.2.a.

Results

SEM-EDX examination showed the formation of Candida albicans biofilm induced by glucose, lactose, soy protein, and iron with similarity in the percentage of the most constituent chemical elements, namely, oxygen, carbon, nitrogen, and phosphorus, and the least were sulfur. The thickest biofilm was found in the induction of iron, glucose, and lactose, and the thinnest was soy protein.

Conclusion

The chemical elements of Candida albicans biofilm induced by four different inducers has the same percentage of the composition of elements, namely, oxygen, carbon, nitrogen, and phosphorus, and the least were sulfur and the thickest biofilm was by the induction of iron, glucose, and lactose, and the thinnest was by soy protein.

Keywords

health - biofilm thickness - Candida albicans - chemical elements - CLSM


Publikationsverlauf

Artikel online veröffentlicht:
20. Mai 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Kemenkes. Infodatin Pusat Data dan Informasi Kementrian Kesehatan RI; 2014:1–6
  • 2 Singh A, Verma R, Murari A, Agrawal A. Oral candidiasis: an overview. J Oral Maxillofac Pathol 2014; 18 (1, Suppl 1): S81-S85
    PubMedSuche in Google Scholar
  • 3 Tsui C, Kong EF, Jabra-Rizk MA. Pathogenesis of Candida albicans biofilm. Pathog Dis 2016; 74 (04) ftw018
    PubMedSuche in Google Scholar
  • 4 Gulati M, Nobile CJ. Candida albicans biofilms: development, regulation, and molecular mechanisms. Microbes Infect 2016; 18 (05) 310-321
    PubMedSuche in Google Scholar
  • 5 Dewi ZY, Nur A, Hertriani T. Efek antibakteri dan penghambatan biofilm ekstrak sereh (Cymbopogon nardus L.) terhadap bakteri Streptococcus mutans . Maj Kedokt Gigi Indones 2015; 20 (02) 136-141
    Suche in Google Scholar
  • 6 Hall CW, Mah TF. Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria. FEMS Microbiol Rev 2017; 41 (03) 276-301
    PubMedSuche in Google Scholar
  • 7 Beitelshees M, Hill A, Jones CH, Pfeifer BA. Phenotypic variation during biofilm formation: implications for anti-biofilm therapeutic design. Materials (Basel) 2018; 11 (07) 1-18
    Suche in Google Scholar
  • 8 Costa OYA, Raaijmakers JM, Kuramae EE. Microbial extracellular polymeric substances: ecological function and impact on soil aggregation. Front Microbiol 2018; 9 (JUL): 1636
    PubMedSuche in Google Scholar
  • 9 Piškur J, Compagno C. (eds) Molecular Mechanisms in Yeast Carbon Metabolism. Berlin, Heidelberg: Springer-Verlag; 2014: 1-326
    Suche in Google Scholar
  • 10 Nuryati A, Huwaina AD. Efektivitas Berbagai Konsentrasi Kacang Kedelai (Glycine max (L. ) Merill) Sebagai Media Alternatif Terhadap Pertumbuhan Jamur Candida albicans. J Teknol Lab. 2015; 5 (01) 5-8
    Suche in Google Scholar
  • 11 Fried HG, Narayanan S, Fallen B. Characterization of a soybean (Glycine max L. Merr.) germplasm collection for root traits. PLoS One 2018; 13 (07) e0200463
    PubMedSuche in Google Scholar
  • 12 Hayrapetyan H, Siezen R, Abee T, Nierop Groot M. Comparative genomics of iron-transporting systems in bacillus cereus strains and impact of iron sources on growth and biofilm formation. Front Microbiol 2016; 7 (JUN): 842
    PubMedSuche in Google Scholar
  • 13 Akay C, Karakis D, Doğan A, Rad AY. Effect of chemical disinfectants on Candida albicans biofilm formation on poly (methyl methacrylate) resin surfaces: a scanning electron microscope study. J Adv Oral Res 2016; 7 (02) 21-26
    Suche in Google Scholar
  • 14 Vila T, Fonseca BB, DA Cunha MML. et al. Candida albicans biofilms: comparative analysis of room-temperature and cryofixation for scanning electron microscopy. J Microsc 2017; 267 (03) 409-419
    PubMedSuche in Google Scholar
  • 15 Koo H, Yamada KM. Dynamic cell-matrix interactions modulate microbial biofilm and tissue 3D microenvironments. Curr Opin Cell Biol 2016; 42: 102-112
    CrossrefPubMedSuche in Google Scholar
  • 16 Tolker-Nielsen T, Molin S. Spatial organization of microbial biofilm communities. Microb Ecol 2000; 40 (02) 75-84
    PubMedSuche in Google Scholar
  • 17 Santana IL, Gonçalves LM, de Vasconcellos AA, da Silva WJ, Cury JA, Del Bel Cury AA. Dietary carbohydrates modulate Candida albicans biofilm development on the denture surface. PLoS One 2013; 8 (05) e64645
    PubMedSuche in Google Scholar
  • 18 Lohse MB, Gulati M, Johnson AD, Nobile CJ. Development and regulation of single- and multi-species Candida albicans biofilms. Nat Rev Microbiol 2018; 16 (01) 19-31
    PubMedSuche in Google Scholar
  • 19 Ikeh M, Ahmed Y, Quinn J. Phosphate acquisition and virulence in human fungal pathogens. Microorganisms 2017; 5 (03) 2-12
    Suche in Google Scholar
  • 20 Mukherjee PK, Zhou G, Munyon R, Ghannoum MA. Candida biofilm: a well-designed protected environment. Med Mycol 2005; 43 (03) 191-208
    PubMedSuche in Google Scholar
  • 21 Horowitz S, Trievel RC. Carbon-oxygen hydrogen bonding in biological structure and function. J Biol Chem 2012; 287 (50) 41576-41582
    PubMedSuche in Google Scholar
  • 22 Ramachandra S, Linde J, Brock M, Guthke R, Hube B, Brunke S. Regulatory networks controlling nitrogen sensing and uptake in Candida albicans . PLoS One 2014; 9 (03) e92734
    PubMedSuche in Google Scholar
  • 23 Lambooij JM, Hoogenkamp MA, Brandt BW, Janus MM, Krom BP. Fungal mitochondrial oxygen consumption induces the growth of strict anaerobic bacteria. Fungal Genet Biol 2017; 109: 1-6
    PubMedSuche in Google Scholar
  • 24 Kraidlova L, Schrevens S, Tournu H, Van Zeebroeck G, Sychrova H, Van Dijck P. Characterization of the Candida albicans amino acid permease family: Gap2 is the only general amino acid permease and Gap4 Is an S-adenosylmethionine (SAM) transporter required for SAM-induced morphogenesis. MSphere 2016; 1 (06) 2-13
    Suche in Google Scholar
  • 25 Amich J, Schafferer L, Haas H, Krappmann S. Regulation of sulphur assimilation is essential for virulence and affects iron homeostasis of the human-pathogenic mould Aspergillus fumigatus . PLoS Pathog 2013; 9 (08) e1003573
    PubMedSuche in Google Scholar
  • 26 Jung J-H, Choi N-Y, Lee S-Y. Biofilm formation and exopolysaccharide (EPS) production by Cronobacter sakazakii depending on environmental conditions. Food Microbiol 2013; 34 (01) 70-80
    PubMedSuche in Google Scholar
  • 27 Jin Y, Samaranayake LP, Samaranayake Y, Yip HK. Biofilm formation of Candida albicans is variably affected by saliva and dietary sugars. Arch Oral Biol 2004; 49 (10) 789-798
    PubMedSuche in Google Scholar
  • 28 Pérez-Giménez J, Mongiardini EJ, Althabegoiti MJ. et al. Soybean lectin enhances biofilm formation by Bradyrhizobium japonicum in the absence of plants. Int J Microbiol 2009; 2009: 719367
    PubMedSuche in Google Scholar