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CC BY 4.0 · European Journal of General Dentistry
DOI: 10.1055/s-0045-1806773
Original Article

Antibacterial Efficacy of Theobroma cacao Mixtures on the Elimination of Streptococcus mutans and Candida albicans

Marisol Rossana Ortega Buitron
1   Department of Odontology, Universidad de Huánuco, Huánuco, Perú
,
Frank Erick Camara Llanos
1   Department of Odontology, Universidad de Huánuco, Huánuco, Perú
,
Deyvis Cano
1   Department of Odontology, Universidad de Huánuco, Huánuco, Perú
,
Héctor Raúl Zacarías Ventura
1   Department of Odontology, Universidad de Huánuco, Huánuco, Perú
,
Lucy Elizabeth Mendoza Vilca
1   Department of Odontology, Universidad de Huánuco, Huánuco, Perú
,
Edith Umasi Ramos 
1   Department of Odontology, Universidad de Huánuco, Huánuco, Perú
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Funding None.
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Abstract

Objectives The aim of the study was to compare the antibacterial efficacy of mixtures based on Theobroma cacao (ThC), ThC plus erythritol, and ThC + erythritol + Psidium guajava (PsG) on Streptococcus mutans and Candida albicans in vitro.

Materials and Methods Pure cacao extract was used through desiccation in alcohol, and guava leaves were macerated in 96% alcohol. Tests were conducted on four groups: pure ThC, ThC sweetened with erythritol, ThC sweetened with erythritol and PsG, and chlorhexidine as a control on strains of S. mutans and C. albicans, at concentrations of 100, 75, 50, and 25%, respectively, evaluated at 24 and 48 hours.

Statistical Analysis ANOVA and Bonferroni tests were performed to compare the time and efficacy on S. mutans and C. albicans with a 95% confidence level. Additionally, a linear regression analysis was conducted using SPSS and InfoStat software.

Results Pure ThC at 100% had greater antibacterial activity than other mixtures against S. mutans, with inhibition halos of 15.34 and 15.16 mm at 24 and 48 hours, respectively, which was almost similar to that observed with 0.12% chlorhexidine (15.7 mm). Furthermore, for C. albicans, pure ThC and ThC + erythritol + PsG at 100% had halos of 10.36 and 10.18 mm, respectively, at both 24 and 48 hours, which were significantly superior to chlorhexidine. Among these mixtures, pure ThC at 100% was the most effective against S. mutans, and pure ThC and ThC + erythritol + PsG mixture at 100% showed antibacterial effectiveness against C. albicans.

Conclusion The 100% concentration mixtures were effective against S. mutans and C. albicans, highlighting the effectiveness of the pure cacao extract. Additionally, the mixture with erythritol and guava extract would be useful as a treatment against C. albicans and also showed similar effectiveness to chlorhexidine against S. mutans.

Keywords

Streptococcus mutans - Candida albicans - Theobroma cacao - antibacterial agent - in vitro


Publikationsverlauf

Artikel online veröffentlicht:
22. April 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • Referencias

  • 1 Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev 1995; 8 (02) 240-259
    PubMedSuche in Google Scholar
  • 2 Wen L, Haddad M, Fernández I. et al. Actividad antifúngica de cuatro plantas usadas en la medicina tradicional peruana. Aislamiento de 3′- formil – 2′,4′,6′ – trihidroxidihidrochalcona, principio activo de Psidium acutangulum. Rev Soc Quím Perú 2011; 77 (03) 199-204
    Suche in Google Scholar
  • 3 Rodríguez Ortega J, Miranda Tarragó J, Morejon Lugones H, Garay Santana JC. Candidiasis de la mucosa bucal. Revisión bibliográfica. Rev Cuba Estomatol 2002; 39 (02) 187-233
    Suche in Google Scholar
  • 4 Estrada Pereira G, Márquez Filiu M, Díaz Fernández J, Agüero Despaigne L. Candidiasis bucal en pacientes con diabetes mellitus Oral Candidiasis in patients with diabetes mellitus Dra. Gladys Aída Estrada Pereira. Medisan (Santiago De Cuba) 2015; 19 (11) 1317-1324
    Suche in Google Scholar
  • 5 Apps.who.int. Organización Mundial Para La Salud. 2014. Accessed February 28, 2025 at: http://apps.who.int/iris/bitstream/10665/95008/1/9789243506098_spa.pdf
  • 6 Ooshima T, Osaka Y, Sasaki H. et al. Caries inhibitory activity of cacao bean husk extract in in-vitro and animal experiments. Arch Oral Biol 2000; 45 (08) 639-645
    PubMedSuche in Google Scholar
  • 7 Beckett S. La Ciencia Del Chocolate. 1st ed. Acribia Editorial; 2008
  • 8 Bodero M. Estudio farmacognósico y actividad antimicrobiana (in vitro) de los extractos fluidos de arrayán y pumín y su aplicación en una pasta dentífrica. Tese. Facultad bioquimica y farmacia. Escuela Politecnica de Chimborazo 2016. Available at: http://dspace.espoch.edu.ec/bitstream/123456789/721/1/56T00239.pdf
  • 9 Correa Jiménez VJ. Efecto inhibitorio del chocolate amargo y chocolate edulcorado con xilitol obtenido de las semillas de cacao (theobrama cacao) frente a Streptococcus mutans. 2017. Accessed February 28, 2025 at: http://www.dspace.uce.edu.ec/bitstream/25000/9577/1/T-UCE-0015-584.pdf
  • 10 Kõljalg S, Smidt I, Chakrabarti A, Bosscher D, Mändar R. Exploration of singular and synergistic effect of xylitol and erythritol on causative agents of dental caries. Sci Rep 2020; 10 (01) 6297
    PubMedSuche in Google Scholar
  • 11 Gutierrez-Montiel D, Guerrero-Barrera AL, Chávez-Vela NA, Avelar-Gonzalez FJ, Ornelas-García IG. Psidium guajava L.: from byproduct and use in traditional Mexican medicine to antimicrobial agent. Front Nutr 2023; 10 (06) 1108306
    PubMedSuche in Google Scholar
  • 12 König A, Schwarzinger B, Stadlbauer V. et al. Guava (Psidium guajava) fruit extract prepared by supercritical CO2 extraction inhibits intestinal glucose resorption in a double-blind, randomized clinical study. Nutrients 2019; 11 (07) 1512
    PubMedSuche in Google Scholar
  • 13 Mandava K, Batchu UR, Kakulavaram S. et al. Design and study of anticaries effect of different medicinal plants against S. mutans glucosyltransferase. BMC Complement Altern Med 2019; 19 (01) 197
    PubMedSuche in Google Scholar
  • 14 Fideles SOM, Ortiz AC, Reis CHB, Buchaim DV, Buchaim RL. Biological properties and antimicrobial potential of cocoa and its effects on systemic and oral health. Nutrients 2023; 15 (18) 1-12
    Suche in Google Scholar
  • 15 Osawa K, Miyazaki K, Shimura S, Okuda J, Matsumoto M, Ooshima T. Identification of cariostatic substances in the cacao bean husk: their anti-glucosyltransferase and antibacterial activities. J Dent Res 2001; 80 (11) 2000-2004
    CrossrefPubMedSuche in Google Scholar
  • 16 Matsumoto M, Tsuji M, Okuda J. et al. Inhibitory effects of cacao bean husk extract on plaque formation in vitro and in vivo. Eur J Oral Sci 2004; 112 (03) 249-252
    PubMedSuche in Google Scholar
  • 17 Smullen J, Koutsou GA, Foster HA, Zumbé A, Storey DM. The antibacterial activity of plant extracts containing polyphenols against Streptococcus mutans . Caries Res 2007; 41 (05) 342-349
    PubMedSuche in Google Scholar
  • 18 García A, Maibely J. Efecto Antifúngico del Extracto Etanólico del Psidium Guajava y Aceite Esencial del Schinus Molle dobre Cándida Albicans ATCC10231 VS Ketoconazol. 2018. Accessed February 28, 2025 at: https://hdl.handle.net/20.500.12692/25500
  • 19 Qian H, Nihorimbere V. Antioxidant power of phytochemicals from Psidium guajava leaf. J Zhejiang Univ Sci 2004; 5 (06) 676-683
    PubMedSuche in Google Scholar
  • 20 de Assis Braga DCA, Gomes PM, Batista MAC. et al. Effects of Psidium guajava L. leaves extract on blood pressure control and IL-10 production in salt-dependent hypertensive rats. Biomed Pharmacother 2022; 155: 113796
    PubMedSuche in Google Scholar