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CC BY 4.0 · Rev Bras Ortop (Sao Paulo) 2024; 59(06): e958-e965
DOI: 10.1055/s-0044-1790217
Artigo Original
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Analysis of Synovial Fluid Metabolic Profile in Patients with Knee Osteoarthritis Using Spectroscopic Magnetic Resonance Metabolomics

Article in several languages: português | English
Paulo Ricardo Picon Alves 
1   Divisão de Ensino e Pesquisa, Instituto Nacional de Traumatologia e Ortopedia Jamil Haddad, Rio de Janeiro, RJ, Brasil
,
Diego Pinheiro Aguiar 
2   Departamento de Farmácia, Faculdade de Ciências Biológicas e Saúde, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
,
Aline Cordeiro Fernandes Ladeira 
1   Divisão de Ensino e Pesquisa, Instituto Nacional de Traumatologia e Ortopedia Jamil Haddad, Rio de Janeiro, RJ, Brasil
,
Gilson Costa dos Santos  Júnior
3   Departamento de Genética, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
4   Laboratório de Metabolômica, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil
,
Eduardo Branco de Sousa 
1   Divisão de Ensino e Pesquisa, Instituto Nacional de Traumatologia e Ortopedia Jamil Haddad, Rio de Janeiro, RJ, Brasil
5   Departamento de Cirurgia Geral e Especializada, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brasil
› Author Affiliations Financial Support The authors declare that they did not receive financial support from agencies in the public, private, or non-profit sectors to conduct the present study.
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Abstract

Objective The present study aimed to evaluate the metabolic profile of synovial fluid in patients with knee osteoarthritis (KOA) and its correlation with clinical data.

Materials and Methods We collected synovial fluid samples from the knees of 50 subjects with KOA undergoing total knee arthroplasty from October 2019 to December 2020. For each patient, we evaluated the clinical data from the medical record, the radiographic osteoarthritis grade, and the preoperative fasting blood glucose levels. The samples underwent metabolomic analysis by 1H magnetic resonance spectroscopy, and we compared the spectra using multivariate and univariate analyses.

Results Most patients were female (66%). The subjects had an average age of 67.96 ± 7.08 years old and an average body mass index (BMI) of 32.51 ± 5.25 kg/m2. Clinical and metabolic evaluations revealed that 88% of patients were hypertensive and presented higher levels of valine, arginine, and citrate than non-hypertensive subjects.

Conclusion Metabolomic analysis of synovial fluid cannot classify osteoarthritis patients per their clinical characteristics.

Keywords

magnetic resonance spectroscopy - metabolomics - osteoarthritis - synovial fluid

Work carried out at the Instituto Nacional de Traumatologia e Ortopedia Jamil Haddad, Rio de Janeiro, RJ, Brazil.




Publication History

Received: 06 December 2023

Accepted: 23 June 2024

Article published online:
21 December 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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