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CC BY 4.0 · Eur J Dent 2025; 19(01): 240-247
DOI: 10.1055/s-0044-1788319
Original Article

Effectiveness of Sectioning Method and Filling Materials on Roughness and Cell Attachments in Root Resection Procedure

Tarek Ashi
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
,
Naji Kharouf    *
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
2   Department of Endodontics and Conservative Dentistry, Faculty of Dental Medicine, University of Strasbourg, Strasbourg, France
,
Olivier Etienne
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
3   Department of Prosthodontics, Faculty of Dental Medicine, University of Strasbourg, Strasbourg, France
4   Pôle de Médecine et Chirurgie Bucco-Dentaire, Hôpital Civil, Hôpitaux Universitaire de Strasbourg, Strasbourg, France
,
Bérangère Cournault
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
3   Department of Prosthodontics, Faculty of Dental Medicine, University of Strasbourg, Strasbourg, France
4   Pôle de Médecine et Chirurgie Bucco-Dentaire, Hôpital Civil, Hôpitaux Universitaire de Strasbourg, Strasbourg, France
,
Pierre Klienkoff
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
4   Pôle de Médecine et Chirurgie Bucco-Dentaire, Hôpital Civil, Hôpitaux Universitaire de Strasbourg, Strasbourg, France
,
Varvara Gribova
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
,
Youssef Haikel   *
1   Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France
2   Department of Endodontics and Conservative Dentistry, Faculty of Dental Medicine, University of Strasbourg, Strasbourg, France
4   Pôle de Médecine et Chirurgie Bucco-Dentaire, Hôpital Civil, Hôpitaux Universitaire de Strasbourg, Strasbourg, France
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Abstract

Objectives The purpose of the present study was to investigate the created roughness and cell attachment of intact teeth (C), obturated teeth with bioceramic (BR), or epoxy resin (AH) after root resection using piezoelectric ultrasonic and carbide bur.

Materials and Methods Three groups of first mandibular premolars were used in the present study: control group (without any preparation or obturation) (C); second group was obturated with an epoxy resin sealer (AH, AH Plus Jet); and finally, the third one was obturated with a bioceramic sealer (BR, BioRoot RCS). All teeth were incubated for 4 months at 37°C. After that, the samples were sectioned using tungsten carbide bur or piezoelectric ultrasonic. Roughness and then cell attachment of periodontal ligament cells on the sectioned surfaces were investigated by profilometer and confocal microscope, respectively.

Statistical Analysis Data were statistically analyzed using one-way analysis of variance.

Results After root resection, no significant difference was found between the roughness among the three groups sectioned using the piezoelectric technique (p > 0.05). In contrast, concerning the sectioned samples by burs, C demonstrated a rougher surface compared with BR (p < 0.05). There was a significant higher cell attachment in BR compared with AH in the piezoelectric groups (p = 0.047), while no statistically significant difference was found between the groups sectioned with bur (p > 0.05).

Conclusion Dentists are now focused on the use of calcium silicate-based sealers due to their bioactivity. The present study advises dentists to use bioceramic sealer which could improve the dentin characteristics which ameliorate the cell attachment.

Keywords

apicoectomy - bioceramic - cell attachment - roughness - tooth resection

* These authors contribute equally to this work.




Publikationsverlauf

Artikel online veröffentlicht:
19. Juli 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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