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CC BY 4.0 · European Journal of General Dentistry 2025; 14(01): 036-043
DOI: 10.1055/s-0044-1787793
Original Article

The Study of Calcium Chloride Effect on Silver Nanoparticles Capping with Roselle Extract Granule against Aggregatibacter actinomycetemcomitans

Thanaphoom Chaiwong
1   Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
2   Phayao Provincial Public Health Office, Phayao, Thailand
,
Ichaya Yiemwattana
3   Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Sasitharee Nathamtong
3   Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Tipruthai Prayoonwong
3   Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Suttimas Yuakyong
4   Research and Innovation Division, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Sirorat Wacharanad
3   Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
› Institutsangaben
Funding This research was supported by the Faculty of Dentistry, Naresuan University.
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Abstract

Objectives The primary aim of this research is to investigate the influence of calcium chloride on the synthesis of silver nanoparticles coated with roselle extract and enclosed within alginate and calcium chloride (SNP-Ro-CaCl2) beads, designated as SNP-Ro-CaCl2 beads. Additionally, the study aims to assess their antimicrobial activity.

Materials and Methods For the preparation of SNP-Ro-CaCl2 beads, SNPs and alginate gel were mixed, followed by dropping in three different concentrations of CaCl2 solution (1%, 3%, and 5% w/v). The morphological structure of the SNP-Ro-CaCl2 beads was analyzed using a stereoscope and scanning electron microscope (SEM). Over a period of 14 days, the release of SNPs was monitored using ultraviolet-visible (UV-Vis) spectroscopy. Additionally, the activity against Aggregatibacter actinomycetemcomitans was evaluated using the disk diffusion technique.

Statistical Analysis The data for this experiment were analyzed using one-way analysis of variance (ANOVA) and Scheffe's method.

Results The results revealed that varying concentrations of calcium chloride had distinct crosslinking effects on alginate, resulting in different voids and porosity within the SNP-Ro-CaCl2 beads. In the SNP-Ro-1% CaCl2 beads, the inner element exhibited higher porosity, facilitating faster activation and greater efficiency in releasing SNPs. Regarding activity against A. actinomycetemcomitans after 14 days, SNP-Ro-1% CaCl2 beads showed a larger inhibition zone diameter compared to other concentrations, while no statistically significant difference in the inhibition zone diameter was observed between SNP-Ro-3% CaCl2 and SNP-Ro-5% CaCl2 beads. Additionally, it was observed that the antimicrobial effectiveness diminished after 17 days through testing of the lifetimes of the three concentrations.

Conclusions This study developed a method for depositing SNP-Ro into alginate gel and crosslinking it with CaCl2 to produce small beads for the sustained release of SNP-Ro in periodontal lesions. Consequently, the SNP-Ro-CaCl2 beads have the potential to be developed as adjunctive locally delivered antimicrobial agents in periodontal therapy.

Keywords

Aggregatibacter actinomycetemcomitans - antimicrobial - calcium chloride - disk diffusion method - microwave-assisted synthesis - periodontal therapy - silver nanoparticles


Publikationsverlauf

Artikel online veröffentlicht:
09. September 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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