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DOI: 10.1055/s-0044-1779705
Original Article

An In Vitro Study to Evaluate the Synergy of Baptisia tinctoria against Salmonella typhi

Soumya Bhattacharyya
1   Department of Materia Medica, D.N. De Homoeopathic Medical College & Hospital, Kolkata, West Bengal, India
,
Rishita Ghosh
2   D.N. De Homoeopathic Medical College & Hospital, Kolkata, West Bengal, India
,
Antara Majumder
3   Department of Medicine, D. N. De Homoeopathic Medical College & Hospital, Kolkata, West Bengal India
,
Pritam Goswami 
4   Department of Repertory, Mahesh Bhattacharyya Homoeopathic Medical College and Hospital, Howrah, West Bengal, India
,
Satadal Das
5   Department of Pathology and Microbiology, Peerless Hospital and B.K. Roy Research Centre, Kolkata, West Bengal, India
› Author Affiliations Funding None.
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Abstract

As a consequence of gradual emergence of anti-microbial resistance, the anti-biogram profile of the microbe Salmonella typhi has undergone substantial changes. Patients frequently utilise homeopathy for the flu season and other serious illnesses. Although the use of Baptisia tinctoria (BT) is extensively described in homeopathic literature and in phytomedicine, scientific exploration has never been done. In this study, we have made an effort to understand the anti-typhoidal effect of BT at different concentrations. S. typhi (ATCC 6539) from a freshly prepared stock culture was used in this experiment. We also procured an international strain of Escherichia coli (ATCC 25922 strain) which was taken as control. The antibiotic susceptibility patterns of the isolates were determined by the Kirby-Bauer disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines and interpretative criteria (National Committee for Clinical Laboratory Standards 2000) using antibiotic discs (Hi Media Laboratory. Pvt. Ltd., Mumbai, Maharashtra, India), namely cefotaxime (30 μg), ceftazidime (30 μg), ciprofloxacin (5 μg), tetracycline (30 μg), chloramphenicol (30 μg), streptomycin (10 μg), ampicillin (10 μg), gentamicin (10 μg), kanamycin (30 μg), nalidixic acid (30 μg) and trimethoprim (5 μg). BT in its 6CH potency showed the greatest growth inhibition zone, not just amidst the homeopathic potency gradient. It has shown the highest growth inhibition zone of 28mm, more than any conventional antibiotic. Ethanolic extract of BT can be considered to combat against array of infections caused by S. typhi species alongside that it can also be considered as adjuvant with conventional antibiotics for better result.

Keywords

Baptisia tinctoria - S. typhi - synergy - anti-microbial resistance - homeopathy

Ethical Approval

IEC was obtained from the Institutional Ethics committee.




Publication History

Article published online:
03 May 2024

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