CC BY-NC-ND 4.0 · PMIO 2018; 5(01): e24-e29
DOI: 10.1055/s-0044-100799
Original Papers
Eigentümer und Copyright ©Georg Thieme Verlag KG 2018

Flavonol Glycosides from Saussurea controversa and Their Efficiency in Experimental Osteomyelitis

Elena Avdeeva
Siberian State Medical University, Tomsk, Russia
,
Elvira Shults
Laboratory of Medicinal Chemistry, Novosibirsk Institute of Organic Chemistry, Siberian Branch, Novosibirsk, Russia
,
Marina Skorokhodova
Siberian State Medical University, Tomsk, Russia
,
Yaroslav Reshetov
Siberian State Medical University, Tomsk, Russia
,
Ekaterina Porokhova
Siberian State Medical University, Tomsk, Russia
,
Irina Sukhodolo
Siberian State Medical University, Tomsk, Russia
,
Efim Krasnov
Siberian State Medical University, Tomsk, Russia
,
Mikhail Belousov
Siberian State Medical University, Tomsk, Russia
› Author Affiliations
Further Information

Publication History

received 27 May 2017
revised 27 December 2017

accepted 28 December 2017

Publication Date:
02 March 2018 (online)

Abstract

Five flavonoids were isolated from leaves of Saussurea controversa, one new flavonoid (5), three described for the first time from the genus Saussurea (1, 2, and 4), and one described previously (3). A number of flavonol glycosides can be easily isolated from the butanol fraction of the ethanol extract from S. controversa. As a result of separating these using column chromatography on silica gel and microcrystalline cellulose, five glycosides of quercetin were isolated in the proportional relationship of 2 (1): 8 (2): 10 (3): 1 (4): 4 (5). The structure of these substances was determined using the method of NMR by conducting various 1H-1H and 1H-13C correlation spectroscopy experiments and by comparing their spectroscopic data with published values, 5,3/,4/-trihydroxyflavone-7-О-α-L-rhamnopyranoside-3-О-β-D-glucopyranosidе (1), 5,7,3/,4/-tetrahydroxyflavone-3-О-β-D-glucopyranoside-(1→6)-β-D-glucopyranoside-(1→6)-О-α-L-rhamnopyranoside (2), 5,7,3/,4/-tetrahydroxyflavone-3-О-β-D-glucopyranoside-(1→6)-О-α-L-rhamnopyranoside (3), 5,3/,4/-trihydroxyflavone-7-О-α-L-rhamnopyranoside-3-О-β-D-xylopyranoside (4), and 5,3/,4/-trihydroxyflavone-7-О-β-D-glucopyranoside-3-O-α-L-rhamnopyranoside-(1→2)-О-β-D-glucopyranoside (5). It was established for the first time that flavonol glycosides from S. controversa stimulate granulopoiesis and lymphopoiesis in the bone marrow of rats, and enhance the reparative process in the affected area in experimental osteomyelitis.

Supporting Information